LOCUS BBa_J70664 5198 bp DNA linear 22-Nov-2024 DEFINITION Test assembly for Grant's parts ACCESSION BBa_J70664 VERSION BBa_J70664.1 FEATURES Location/Qualifiers misc_feature 7..19 /label=PsrI Site misc_feature 1..19 /label=Left BioScaffold Padding misc_feature 879..896 /label=Right BioScaffold Padding misc_feature 879..891 /label=PsrI Site promoter 20..68 /label=Lambda Promoter RBS 77..88 /label=Elowitz RBS CDS 95..800 /label=RFP misc_feature 809..896 /label=Truncated Terminator misc_feature 1..896 /label=BBa_M12100 misc_feature 905..921 /label=rbs misc_feature 2179..2195 /label=rbs CDS 922..2178 /label=vioA CDS 2196..5192 /label=vioB misc_feature 905..5198 /label=BBa_J70638 ORIGIN 1 cagtcagaac ctcctctact aacaccgtgc gtgttgacta ttttacctct ggcggtgata 61 atggttgcta ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa 121 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat 181 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac 241 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc 301 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga 361 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca 421 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt 481 cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg 541 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga 601 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct 661 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac 721 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg 781 atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtact 901 agagttaagg aggtaaaaaa aatgaaacat tcttccgata tctgcattgt tggtgctggt 961 atttctggtt tgacgtgcgc aagccatctg ctggacagcc cggcatgccg tggtctgagc 1021 ctgcgtatct ttgacatgca gcaagaagcc ggtggccgta tccgcagcaa aatgctggat 1081 ggtaaggcaa gcattgaact gggcgcaggt cgctactccc ctcagttgca cccgcatttc 1141 caaagcgcaa tgcagcacta tagccaaaag agcgaagtct atccgttcac ccagttgaag 1201 ttcaaatctc acgtgcagca aaagctgaag cgcgccatga atgaactgtc cccgcgtctg 1261 aaagagcatg gtaaagagag ctttttgcag tttgtcagcc gttatcaagg tcacgatagc 1321 gcggttggta tgatccgctc tatgggttac gacgcactgt tcctgccgga tatcagcgca 1381 gaaatggcct acgacattgt gggtaagcac ccggagatcc agagcgtgac ggacaacgac 1441 gcgaaccaat ggtttgcagc ggaaacgggc tttgctggtc tgattcaggg catcaaggct 1501 aaggttaagg cggcaggtgc gcgttttagc ctgggttatc gtctgctgag cgtccgtacc 1561 gacggtgacg gctacctgct gcaactggca ggtgacgacg gctggaaact ggagcaccgt 1621 acccgccatc tgattctggc gattccgccg agcgcgatgg cgggtttgaa tgttgatttt 1681 ccagaagcct ggtccggtgc gcgctatggc agcctgccgc tgtttaaggg ctttctgacg 1741 tacggtgagc cgtggtggtt ggactacaaa ctggacgatc aggtgctgat tgttgacaac 1801 ccgctgcgca aaatctattt caaaggcgat aagtacctgt tcttctatac cgatagcgag 1861 atggcgaatt actggcgcgg ttgtgtcgcg gagggcgagg acggttacct ggagcaaatt 1921 cgcacccatt tggctagcgc actgggtatc gtccgtgaac gtatcccgca accgctggca 1981 cacgttcaca agtattgggc gcacggcgtt gagttttgcc gtgattctga tattgaccac 2041 ccgagcgcac tgtctcatcg cgacagcggt atcatcgcgt gctccgatgc gtacacggag 2101 cattgtggtt ggatggaggg cggtctgctg agcgcccgtg aggcaagccg tctgctgttg 2161 cagcgtatcg ccgcgtgatt aaggaggtaa aaaaaatgag cattctggat ttcccgcgta 2221 tccacttccg tggctgggcc cgtgtcaatg cgccgaccgc gaaccgcgat ccgcacggcc 2281 acatcgatat ggccagcaat accgtggcga tggcgggtga gccgttcgac ctggcacgcc 2341 atcctacgga gttccaccgt cacctgcgct ccctgggtcc gcgcttcggc ttggatggtc 2401 gtgctgaccc ggaaggcccg ttcagcctgg ccgagggcta caacgctgcc ggtaacaacc 2461 acttttcgtg ggagagcgca accgttagcc acgtgcaatg ggatggcggt gaggcggatc 2521 gtggtgacgg tctggtcggt gctcgtttgg cactgtgggg tcactacaat gattatctgc 2581 gtaccacctt caatcgtgct cgttgggtcg acagcgaccc gacgcgccgt gacgctgcac 2641 aaatctatgc gggccaattc accattagcc cggctggtgc cggtccgggt acgccgtggc 2701 tgtttacggc agacattgat gatagccatg gtgcacgttg gacgcgtggc ggccacattg 2761 cagagcgtgg cggccacttc ttggatgaag agtttggtct ggcacgcctg tttcagttct 2821 ctgtgccgaa agatcaccca cattttctgt ttcacccggg tccgtttgat tccgaggcct 2881 ggcgtcgtct gcaattggct ctggaggatg acgacgttct gggtctgacc gtgcaatatg 2941 cgttgttcaa tatgagcacc ccgcctcagc cgaacagccc ggtttttcac gatatggtcg 3001 gtgttgtcgg tctgtggcgt cgtggtgaac tggcgagcta cccggctggt cgtctgctgc 3061 gtccgcgtca accgggtctg ggtgacctga ccctgcgcgt caacggtggt cgcgttgcgc 3121 tgaatttggc gtgtgccatt ccgttcagca ctcgtgccgc gcagccaagc gcaccggacc 3181 gcctgacccc ggacctgggt gccaaactgc cgctgggcga tctgctgctg cgtgatgagg 3241 acggcgcact gttggcacgt gtgccgcagg ctctgtacca agactattgg acgaatcacg 3301 gtattgtgga cctgccgctg ctgcgcgaac cgcgtggtag cttgaccctg agcagcgaac 3361 tggcggagtg gcgtgagcaa gactgggtca cccaaagcga cgcgtctaac ctgtacctgg 3421 aggcaccgga tcgccgtcac ggtcgctttt tccctgagag catcgcgctg cgcagctact 3481 ttcgcggtga agcgcgtgcg cgtccggata tcccgcatcg tatcgagggc atgggcctgg 3541 tcggcgtcga atctcgtcag gatggcgacg ctgcggaatg gcgtctgacg ggtctgcgtc 3601 cgggtccggc acgcattgtt ctggacgatg gtgccgaggc gatccctctg cgtgttctgc 3661 ctgacgattg ggcgctggat gacgcgaccg tcgaagaagt ggattacgcc tttttgtacc 3721 gccacgttat ggcgtattac gagctggtgt atccattcat gagcgacaag gtgttttccc 3781 tggctgatcg ttgcaaatgt gaaacgtacg cacgtctgat gtggcagatg tgtgatccgc 3841 agaaccgcaa caagtcctat tacatgccga gcacccgcga actgtcggca ccgaaagctc 3901 gtttgttctt gaagtatctg gcccacgtgg aaggccaggc acgcctgcaa gcacctccgc 3961 cagcgggtcc ggcacgcatt gaatctaaag cccagttggc ggcagagctg cgtaaagccg 4021 tcgacctgga gctgtctgtg atgctgcaat acctgtacgc ggcgtatagc attccgaact 4081 atgcacaggg ccaacaacgt gttcgtgacg gtgcgtggac cgccgagcag ctgcaactgg 4141 cgtgcggtag cggtgaccgt cgccgtgatg gcggtattcg tgcagcactg ctggaaattg 4201 ctcatgaaga aatgattcat tacctggtcg ttaacaacct gctgatggcc ctgggcgagc 4261 cgttctacgc gggtgtcccg ctgatgggcg aagcggcacg tcaggcgttt ggcctggaca 4321 ccgagttcgc tctggaaccg tttagcgaaa gcacgctggc acgttttgtt cgtctggaat 4381 ggccgcactt tatcccagca ccgggcaaat ccatcgcgga ctgctatgcc gccattcgtc 4441 aggcgttttt ggatctgccg gacttgtttg gtggcgaggc aggtaagcgt ggcggtgaac 4501 accacctgtt cctgaatgag ctgaccaacc gtgcgcatcc gggttatcaa ctggaagttt 4561 tcgatcgcga ctcggcgctg tttggtattg catttgtgac cgatcagggc gaaggtggcg 4621 ctctggacag cccgcactac gaacatagcc attttcaacg tctgcgtgaa atgagcgcgc 4681 gtatcatggc tcaaagcgca ccgttcgaac cggcgctgcc ggcgttgcgt aatccggttc 4741 tggatgagag cccgggttgc caacgtgtcg cagacggtcg tgcgcgtgcg ctgatggcat 4801 tgtaccaagg cgtttatgag ctgatgtttg cgatgatggc gcagcacttc gccgtgaaac 4861 cgctgggtag cttgcgtcgc agccgcctga tgaacgcagc aatcgatctg atgaccggtc 4921 tgttgcgtcc gctgagctgc gcgctgatga acctgccaag cggcatcgcc ggtcgcacgg 4981 ccggtccgcc gctgccgggt ccggttgaca cccgtagcta tgacgactac gcgctgggct 5041 gtcgcatgct ggcacgccgt tgcgagcgtc tgctggagca ggcgagcatg ctggaaccgg 5101 gttggctgcc ggatgcgcag atggagctgc tggatttcta tcgtcgccaa atgctggact 5161 tggcgtgcgg caaactgagc cgcgaggcct aaggatct //