BBa_J70655 1 BBa_J70655 RFP optimized for expression in E. coli and M. florum 2010-12-01T12:00:00Z 2015-05-08T01:08:25Z Design, DNA2.0 Codon optimized version of red fluorescent protein, optimized for dual expression in E. coli and M. florum. Includes an RBS and CDS. Designed to express well in either species. Designed with Gene Designer and made by DNA 2.0, provided in their pJ201 plasmid with Kan resistance. false false _42_41_48_1_ 0 6 48 Not in stock false Restriction site elimination, coding bias, elimination of CGG codon. false Tom Knight annotation2114854 1 MF-RED range2114854 1 52 699 BBa_J70300 1 BBa_J70300 Strong Me. florum promoter 2009-03-28T12:00:00Z 2015-05-08T01:08:20Z 53556 53678 approximately from NC_006055 Promoter from the highly expressed pdh e1 gene (mfl039) of Me. florum. Contains three overlapping promoters with near consensus sequence and an UP sequence. false false _42_41_48_1_ 0 6 48 Not in stock false None. false Tom Knight annotation2002222 1 -10 range2002222 1 77 82 annotation2002225 1 -35 range2002225 1 43 48 annotation2002224 1 -10 range2002224 1 67 73 annotation2002223 1 -35 range2002223 1 54 60 annotation2002226 1 t-c mutation range2002226 1 16 16 BBa_J70666 1 BBa_J70666 MF optimized RFP reporter, MF promoter 2011-04-04T11:00:00Z 2015-05-08T01:08:26Z Composite J70300, J70655 false false _41_48_1_ 0 6 48 Not in stock false None false Tom Knight component2115787 1 BBa_J70655 component2115785 1 BBa_J70300 annotation2115787 1 BBa_J70655 range2115787 1 91 789 annotation2115785 1 BBa_J70300 range2115785 1 1 82 BBa_J70300_sequence 1 cagttaaaaacattccagataagtttgcattaaaagaaatatttggtgataactttgaagcagaagatattttgaatttaat BBa_J70655_sequence 1 tcacacaggaaagactagatggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaatttgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggacctttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatataccagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgttacagttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcgatggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaaaggagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaaaagcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattgttgaacagtatgaaagagcagaaggaagacattctacaggagcataataa BBa_J70666_sequence 1 cagttaaaaacattccagataagtttgcattaaaagaaatatttggtgataactttgaagcagaagatattttgaatttaattactagagtcacacaggaaagactagatggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaatttgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggacctttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatataccagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgttacagttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcgatggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaaaggagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaaaagcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattgttgaacagtatgaaagagcagaaggaagacattctacaggagcataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z