BBa_J70667 1 BBa_J70667 MF optimized RFP reporter, MF heat induced promoter 2011-04-04T11:00:00Z 2015-05-08T01:08:26Z Composite J70301, J70655 false false _41_48_1_ 0 6 48 Not in stock false None false Tom Knight component2115793 1 BBa_J70655 component2115791 1 BBa_J70301 annotation2115793 1 BBa_J70655 range2115793 1 120 818 annotation2115791 1 BBa_J70301 range2115791 1 1 111 BBa_J70301 1 BBa_J70301 Heat shock inducible promoter for Me. florum 2009-03-29T12:00:00Z 2015-05-08T01:08:20Z PCR from lon protease promoter of NC_006055. Promoter inhibited by the gram + hrcA gene product in normal temperature conditions. Promoter from the lon protease, false false _42_41_48_1_ 0 6 48 Not in stock false Low gc content, difficult PCR primers false Tom Knight annotation2002228 1 -35 range2002228 1 73 79 annotation2002227 1 -10 range2002227 1 94 100 annotation2002229 1 hrcA binding site range2002229 1 53 80 BBa_J70655 1 BBa_J70655 RFP optimized for expression in E. coli and M. florum 2010-12-01T12:00:00Z 2015-05-08T01:08:25Z Design, DNA2.0 Codon optimized version of red fluorescent protein, optimized for dual expression in E. coli and M. florum. Includes an RBS and CDS. Designed to express well in either species. Designed with Gene Designer and made by DNA 2.0, provided in their pJ201 plasmid with Kan resistance. false false _42_41_48_1_ 0 6 48 Not in stock false Restriction site elimination, coding bias, elimination of CGG codon. false Tom Knight annotation2114854 1 MF-RED range2114854 1 52 699 BBa_J70301_sequence 1 caaataacggataattataaaataaaacaaacctgataaaggtttttttatttttagcactctactattgtaattgctaaacaatatgataatatatatttaaccacatag BBa_J70655_sequence 1 tcacacaggaaagactagatggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaatttgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggacctttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatataccagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgttacagttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcgatggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaaaggagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaaaagcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattgttgaacagtatgaaagagcagaaggaagacattctacaggagcataataa BBa_J70667_sequence 1 caaataacggataattataaaataaaacaaacctgataaaggtttttttatttttagcactctactattgtaattgctaaacaatatgataatatatatttaaccacatagtactagagtcacacaggaaagactagatggctagtagtgaggatataatcaaagagtttatgcgttttaaagttcacatggaaggttcagtaaacggacatgaatttgaaattgagggagaaggagagggtcgtccgtacgaaggtacacaaacagcaaagttaaaagtaacaaaaggaggacctttaccatttgcatgggatattctttctccacaatttatgtatggtagtaaagcgtatgttaaacacccggcagatataccagactatttaaagttgagttttccggaaggattcaaatgggaacgtgttatgaattttgaagatggtggtgttgttacagttactcaagatagtagcttacaggatggtgaatttatttacaaagtaaaattacgtggtactaacttcccgagcgatggtccagtaatgcaaaagaaaactatgggatgggaggctagttctgaacgtatgtatcctgaagacggtgctttaaaaggagaaattaaacaacgtttgaaacttaaagatggtggacactacgatgcagaagttaaaactacatataaagctaaaaagcctgttcagcttccgggtgcttataatgtgaatataaaattagatatcacaagtcataacgaagattatactattgttgaacagtatgaaagagcagaaggaagacattctacaggagcataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z