BBa_J72016
1
BBa_J72016
{Bmll56} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension PCR (Wobble construction)
===Wobble construction of Bmll56 {rbs_at_10000.6XHis>}===
<pre>
Wobble mll56/mll57 (88, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll56 {rbs_at_10000.6XHis>}
----
mll56 Forward wobble for {rbs_at_10000.6XHis>} (Bmll56)<br>
CCATAGAATTCATGAGATCTCTACAACACCTAGCATCTAAGAAGTTACATATGGATTC<br>
mll57 Reverse wobble for {rbs_at_10000.6XHis>} (Bmll56)<br>
CGTTAGGATCCGTGATGGTGATGATGGTGAGAATCCATATGTAACTTCTTAG<br>
{rbs_at_1000.6XHis} RBS with an N-terminal 6XHis tag.
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073900
1
6XHis tag
range2073900
1
45
60
annotation2073901
1
Rbs_at_1000
range2073901
1
6
36
BBa_J72016_sequence
1
gatctctacaacacctagcatctaagaagttacatatggattctcaccatcatcaccatcacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z