BBa_J72016 1 BBa_J72016 {Bmll56} in BBb 2010-07-14T11:00:00Z 2015-05-08T01:08:26Z Overlap Extension PCR (Wobble construction) ===Wobble construction of Bmll56 {rbs_at_10000.6XHis>}=== <pre> Wobble mll56/mll57 (88, EcoRI/BamHI) Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll56 {rbs_at_10000.6XHis>} ---- mll56 Forward wobble for {rbs_at_10000.6XHis>} (Bmll56)<br> CCATAGAATTCATGAGATCTCTACAACACCTAGCATCTAAGAAGTTACATATGGATTC<br> mll57 Reverse wobble for {rbs_at_10000.6XHis>} (Bmll56)<br> CGTTAGGATCCGTGATGGTGATGATGGTGAGAATCCATATGTAACTTCTTAG<br> {rbs_at_1000.6XHis} RBS with an N-terminal 6XHis tag. false false _171_ 0 4745 271 It's complicated false N/A false Jennifer Brophy annotation2073900 1 6XHis tag range2073900 1 45 60 annotation2073901 1 Rbs_at_1000 range2073901 1 6 36 BBa_J72016_sequence 1 gatctctacaacacctagcatctaagaagttacatatggattctcaccatcatcaccatcacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z