BBa_J72017 1 BBa_J72017 {Bmll38} in BBb 2010-07-14T11:00:00Z 2015-05-08T01:08:26Z Overlap Extension PCR (Wobble Construction) ===Wobble construction of Bmll38 {rbs_at_10000.AP>}=== <pre> Wobble mll38/mll39 (108, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll38 {rbs_at_10000.AP>} ---- mll38 Forward wobble for {rbs_at_10000.AP>} (Bmll38) CCATAGAATTCATGAGATCTCACAAACTGATACAAACGGCGGGGAAATCATGGGCCTGAACGATATTTTTG mll39 Reverse wobble for {rbs_at_10000.AP>} (Bmll38) CGTTAGGATCCTTCATGCCATTCAATTTTCTGCGCTTCAAAAATATCGTTCAGGCCCATG </pre> {rbs_at_10000.avi} N terminal avi tag: RBS with an avi tag. false false _171_ 0 4745 271 It's complicated false N/A false Jennifer Brophy annotation2073904 1 RBS at 10000 range2073904 1 6 35 BBa_J72017_sequence 1 gatctcacaaactgatacaaacggcggggaaatcatgggcctgaacgatatttttgaagcgcagaaaattgaatggcatgaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z