BBa_J72019
1
BBa_J72019
{Bmll54} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension PCR (Wobble Construction)
===Wobble construction of Bmll54 {rbs_at_10000.FLAG>}===
<pre>
Wobble mll54/mll55 (97, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll54 {rbs_at_10000.FLAG>}
----
mll54 Forward wobble for {rbs_at_10000.FLAG>} (Bmll54)
CCATAGAATTCATGAGATCTGGGACAATCCCGGTCTTTAAGAACAGGAATTACATGTCTGAC
mll55 Reverse wobble for {rbs_at_10000.FLAG>} (Bmll54)
CGTTAGGATCCCTTGTCGTCGTCATCCTTGTAATCGTCAGACATGTAATTCCTG
</pre>
{rbs_at_10000.FLAG} N terminal flag tag: RBS with a FLAG tag.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073908
1
FLAG tag
range2073908
1
42
69
annotation2073907
1
RBS at 10000
range2073907
1
6
39
BBa_J72019_sequence
1
gatctgggacaatcccggtctttaagaacaggaattacatgtctgacgattacaaggatgacgacgacaagg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z