BBa_J72020
1
BBa_J72020
{Bmll50} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension PCR (Wobble Construction)
===Wobble construction of Bmll50 {rbs_at_10000.HA>}===
<pre>
Wobble mll50/mll51 (100, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll50 {rbs_at_10000.HA>}
----
mll50 Forward wobble for {rbs_at_10000.HA>} (Bmll50)
CCATAGAATTCATGAGATCTTAAGCACCCATAGAATACACACCTGGAGGAATAATGTCTTACC
mll51 Reverse wobble for {rbs_at_10000.HA>} (Bmll50)
CGTTAGGATCCCCCAGCGTAGTCTGGGACGTCGTATGGGTAAGACATTATTCCTC
</pre>
{rbs_at_10000.HA} N terminal HA tag: RBS with an HA tag.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073910
1
HA tag
range2073910
1
42
75
annotation2073909
1
RBS at 10000
range2073909
1
6
39
BBa_J72020_sequence
1
gatcttaagcacccatagaatacacacctggaggaataatgtcttacccatacgacgtcccagactacgctgggg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z