BBa_J72022 1 BBa_J72022 {Bmll44} in BBb 2010-07-14T11:00:00Z 2015-05-08T01:08:26Z Overlap extension (Wobble Construction) ===Wobble construction of Bmll44 {rbs_at_10000.myc>}=== <pre> Wobble mll44/mll45 (102, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll44 {rbs_at_10000.myc>} ---- mll44 Forward wobble for {rbs_at_10000.myc>} (Bmll44) CCATAGAATTCATGAGATCTACGCAATATCACACGGAACCGGAGGGCTAACTCGCATGTCTGAAC mll45 Reverse wobble for {rbs_at_10000.myc>} (Bmll44) CGTTAGGATCCCAGATCCTCTTCTGAGATGAGTTTTTGTTCAGACATGCGAGTTAG </pre> {rbs_at_10000.myc} N terminal MYC tag: RBS with a myc tag. ---- This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _171_ 0 4745 271 It's complicated false N/A false Jennifer Brophy annotation2073917 1 MYC tag range2073917 1 44 77 annotation2073916 1 RBS at 10000 range2073916 1 6 40 BBa_J72022_sequence 1 gatctacgcaatatcacacggaaccggagggctaactcgcatgtctgaacaaaaactcatctcagaagaggatctgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z