BBa_J72023 1 BBa_J72023 {Bmll34} in BBb 2010-07-14T11:00:00Z 2015-05-08T01:08:26Z Overlap Extension (Wobble Construction) ===Wobble construction of Bmll34 {rbs_at_10000.S>}=== <pre> Wobble mll34/mll35 (112, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll34 {rbs_at_10000.S>} ---- mll34 Forward wobble for {rbs_at_10000.S>} (Bmll34) CCATAGAATTCATGAGATCTGAAAAGGAACCCACGCCAAGTCCAGGACACGCCATGAAAGAAACCGCTG mll35 Reverse wobble for {rbs_at_10000.S>} (Bmll34) CGTTAGGATCCGGAATCCATGTGCTGACGTTCGAATTTAGCAGCAGCGGTTTCTTTCATGG </pre> {rbs_at_10000.S} N terminal S tag with RBS. false false _171_ 0 4745 271 It's complicated false N/A false Jennifer Brophy annotation2073919 1 S tag range2073919 1 42 87 annotation2073918 1 RBS at 10000 range2073918 1 6 39 BBa_J72023_sequence 1 gatctgaaaaggaacccacgccaagtccaggacacgccatgaaagaaaccgctgctgctaaattcgaacgtcagcacatggattccg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z