BBa_J72024
1
BBa_J72024
{Bmll48} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension (Wobble Construction)
===Wobble construction of Bmll48 {rbs_at_10000.Strep>}===
<pre>
Wobble mll48/mll49 (101, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll48 {rbs_at_10000.Strep>}
----
mll48 Forward wobble for {rbs_at_10000.Strep>} (Bmll48)
CCATAGAATTCATGAGATCTTACAACTACACACTATCTCATACGGAGCCCTAACATGTCTGACAC
mll49 Reverse wobble for {rbs_at_10000.Strep>} (Bmll48)
CGTTAGGATCCTTTTTCGAACTGCGGGTGGCTCCAGGTGTCAGACATGTTAGGGC
</pre>
{rbs_at_10000.Strep} N terminal strep tag with RBS.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073921
1
Strep Tag
range2073921
1
43
76
annotation2073920
1
RBS at 10000
range2073920
1
6
40
BBa_J72024_sequence
1
gatcttacaactacacactatctcatacggagccctaacatgtctgacacctggagccacccgcagttcgaaaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z