BBa_J72024 1 BBa_J72024 {Bmll48} in BBb 2010-07-14T11:00:00Z 2015-05-08T01:08:26Z Overlap Extension (Wobble Construction) ===Wobble construction of Bmll48 {rbs_at_10000.Strep>}=== <pre> Wobble mll48/mll49 (101, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll48 {rbs_at_10000.Strep>} ---- mll48 Forward wobble for {rbs_at_10000.Strep>} (Bmll48) CCATAGAATTCATGAGATCTTACAACTACACACTATCTCATACGGAGCCCTAACATGTCTGACAC mll49 Reverse wobble for {rbs_at_10000.Strep>} (Bmll48) CGTTAGGATCCTTTTTCGAACTGCGGGTGGCTCCAGGTGTCAGACATGTTAGGGC </pre> {rbs_at_10000.Strep} N terminal strep tag with RBS. ---- This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _171_ 0 4745 271 It's complicated false N/A false Jennifer Brophy annotation2073921 1 Strep Tag range2073921 1 43 76 annotation2073920 1 RBS at 10000 range2073920 1 6 40 BBa_J72024_sequence 1 gatcttacaactacacactatctcatacggagccctaacatgtctgacacctggagccacccgcagttcgaaaaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z