BBa_J72025
1
BBa_J72025
{Bmll52} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension PCR (Wobble Construction)
===Wobble construction of Bmll52 {rbs_at_10000.T7>}===
<pre>
Wobble mll52/mll53 (98, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll52 {rbs_at_10000.T7>}
----
mll52 Forward wobble for {rbs_at_10000.T7>} (Bmll52)
CCATAGAATTCATGAGATCTGTACCAAATTAAAAAAATCAAAACGGGGTAAGATATGGCTTC
mll53 Reverse wobble for {rbs_at_10000.T7>} (Bmll52)
CGTTAGGATCCACCCATTTGCTGACCGCCAGTCATAGAAGCCATATCTTACCCC
</pre>
{rbs_at_10000.T7} N terminal T7 tag with RBS.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073923
1
T7 tag
range2073923
1
43
73
annotation2073922
1
RBS at 10000
range2073922
1
6
40
BBa_J72025_sequence
1
gatctgtaccaaattaaaaaaatcaaaacggggtaagatatggcttctatgactggcggtcagcaaatgggtg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z