BBa_J72026 1 BBa_J72026 {Bmll36} in BBb 2010-07-14T11:00:00Z 2015-05-08T01:08:26Z Overlap Extension PCR (Wobble Construction) ===Wobble construction of Bmll36 {rbs_at_10000.V5>}=== <pre> Wobble mll36/mll37 (109, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll36 {rbs_at_10000.V5>} ---- mll36 Forward wobble for {rbs_at_10000.V5>} (Bmll36) CCATAGAATTCATGAGATCTGAAGCAATATCTTCACGTATAAGGAGGTATTTCATGGGTAAGCCTATC mll37 Reverse wobble for {rbs_at_10000.V5>} (Bmll36) CGTTAGGATCCCGTAGAATCGAGACCCAGCAGAGGGTTAGGGATAGGCTTACCCATGAAATAC </pre> {rbs_at_10000.V5} N terminal V5 tag with RBS. ---- This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _171_ 0 4745 271 It's complicated false N/A false Jennifer Brophy annotation2073927 1 V5 tag range2073927 1 42 84 annotation2073928 1 RBS at 10000 range2073928 1 6 39 BBa_J72026_sequence 1 gatctgaagcaatatcttcacgtataaggaggtatttcatgggtaagcctatccctaaccctctgctgggtctcgattctacgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z