BBa_J72026
1
BBa_J72026
{Bmll36} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension PCR (Wobble Construction)
===Wobble construction of Bmll36 {rbs_at_10000.V5>}===
<pre>
Wobble mll36/mll37 (109, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll36 {rbs_at_10000.V5>}
----
mll36 Forward wobble for {rbs_at_10000.V5>} (Bmll36)
CCATAGAATTCATGAGATCTGAAGCAATATCTTCACGTATAAGGAGGTATTTCATGGGTAAGCCTATC
mll37 Reverse wobble for {rbs_at_10000.V5>} (Bmll36)
CGTTAGGATCCCGTAGAATCGAGACCCAGCAGAGGGTTAGGGATAGGCTTACCCATGAAATAC
</pre>
{rbs_at_10000.V5} N terminal V5 tag with RBS.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073927
1
V5 tag
range2073927
1
42
84
annotation2073928
1
RBS at 10000
range2073928
1
6
39
BBa_J72026_sequence
1
gatctgaagcaatatcttcacgtataaggaggtatttcatgggtaagcctatccctaaccctctgctgggtctcgattctacgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z