BBa_J72027
1
BBa_J72027
{Bmll46} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension PCR (Wobble Construction)
===Wobble construction of Bmll46 {rbs_at_10000.VSV>}===
<pre>
Wobble mll46/mll47 (102, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll46 {rbs_at_10000.VSV>}
----
mll46 Forward wobble for {rbs_at_10000.VSV>} (Bmll46)
CCATAGAATTCATGAGATCTCAATAGAACATTAGATCAGAACGCGAAAGGGAACAATGTACACC
mll47 Reverse wobble for {rbs_at_10000.VSV>} (Bmll46)
CGTTAGGATCCCTTTCCCAGCCTGTTCATCTCTATATCGGTGTACATTGTTCCCTTTC
</pre>
{rbs_at_10000.VSV} N terminal VSV tag with RBS.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073930
1
VSV Tag
range2073930
1
44
77
annotation2073929
1
RBS at 10000
range2073929
1
6
41
BBa_J72027_sequence
1
gatctcaatagaacattagatcagaacgcgaaagggaacaatgtacaccgatatagagatgaacaggctgggaaagg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z