BBa_J72030
1
BBa_J72030
{Bmll62} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension PCR (Wobble Construction)
===Wobble construction of Bmll62 {<E!}===
<pre>
Wobble mll62/mll63 (73, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll62 {<E!}
----
mll62 Forward wobble for {<E!} (Bmll62)
CCATAGAATTCATGAGATCTGGTGCTCCGGTTCCATATCCAGATCCACTG
mll63 Reverse wobble for {<E!} (Bmll62)
CGTTAGGATCCTTAACGTGGTTCCAGTGGATCTGGATATGG
</pre>
{E!} C terminal E tag.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073933
1
E tag
range2073933
1
6
45
BBa_J72030_sequence
1
gatctggtgctccggttccatatccagatccactggaaccacgttaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z