BBa_J72035
1
BBa_J72035
{Bmll60} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Overlap Extension PCR (Wobble Construction)
===Wobble construction of Bmll60 {<V5!}===
<pre>
Wobble mll60/mll61 (76, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll60 {<V5!}
----
mll60 Forward wobble for {<V5!} (Bmll60)
CCATAGAATTCATGAGATCTGGTAAGCCTATCCCTAACCCTCTGCTGGGTC
mll61 Reverse wobble for {<V5!} (Bmll60)
CGTTAGGATCCTTACGTAGAATCGAGACCCAGCAGAGGGTTAG
</pre>
{V5!} C terminal V5 tag.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
annotation2073940
1
V5 tag
range2073940
1
6
48
BBa_J72035_sequence
1
gatctggtaagcctatccctaaccctctgctgggtctcgattctacgtaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z