BBa_J72037
1
BBa_J72037
{Bmll5} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
PCR
Construction of MED7 Basic Part Bmll5
PCR MLL3/MLL78 on pBca9523-Bmll3 (324bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-Bmll5
-------------------------------------
MLL3 Forward Biobricking of Bmll5 CCATAGAATTCATGAGATCTATGAAAAAATCTACCGAAA
MLL78 Reverse Biobricking of Bmll5 CGTTAGGATCCAGAGGTCAGTTTGTCGTGAACCTGTTTG
{Med7DNDc102-205!} Protein 7 of the Mediator Complex (MED7) with a stop codon. This version of MED7 was made to be tagged N-terminally.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
BBa_J72037_sequence
1
gatctatgaaaaaatctaccgaaaacgaatctaccaactaccagtacaaaatccaggaactgcgtaaactgctgaaatctctgctgctgaactacctggaactgatcggtgttctgtctatcaacccggacatgtacgaacgtaaagttgaaaacatccgtaccatcctggttaacatccaccacctgctgaacgaataccgtccgcaccagtctcgtgaatctctgatcatgctgctggaagaacagctggaatacaaacgtggtgaaatccgtgaaatcgaacaggtttgcaaacaggttcacgacaaactgacctcttaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z