BBa_J72039 1 BBa_J72039 {Bmll3} in BBb 2010-07-14T11:00:00Z 2015-05-08T01:08:26Z Polymerase Chain Assembly ===Gene Synthesis of Bmll3 {rbs_at_10000.MED7DNDC102-205>}=== <pre> Pool mll4 through mll15, assemble by PCA PCR mll4/mll15 on PCA reaction (384, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll3 {rbs_at_10000.MED7DNDC102-205>} ---- mll4 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) CCATAGAATTCATGAGATCTCAGCTAAAATCAACGTATTAAG mll5 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) TTTCGGTAGATTTTTTCATCGCTATGGAGTGCCTTAATACGTTGATTTTAGC mll6 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) GATGAAAAAATCTACCGAAAACGAATCTACCAACTACCAGTACAAAATCCAG mll7 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) AGCAGCAGAGATTTCAGCAGTTTACGCAGTTCCTGGATTTTGTACTGGTAGT mll8 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) CTGCTGAAATCTCTGCTGCTGAACTACCTGGAACTGATCGGTGTTCTGTCTA mll9 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) TTCAACTTTACGTTCGTACATGTCCGGGTTGATAGACAGAACACCGATCAGT mll10 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) TGTACGAACGTAAAGTTGAAAACATCCGTACCATCCTGGTTAACATCCACCA mll11 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) GAGACTGGTGCGGACGGTATTCGTTCAGCAGGTGGTGGATGTTAACCAGGAT mll12 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) ATACCGTCCGCACCAGTCTCGTGAATCTCTGATCATGCTGCTGGAAGAACAG mll13 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) ATTTCACGGATTTCACCACGTTTGTATTCCAGCTGTTCTTCCAGCAGCATGA mll14 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) CGTGGTGAAATCCGTGAAATCGAACAGGTTTGCAAACAGGTTCACGACAAAC mll15 PCA assembly of {rbs_at_10000.MED7DNDC102-205>} (Bmll3) CGTTAGGATCCTTAAGAGGTCAGTTTGTCGTGAACCTGTTTG </pre> {rbs_at_10000.MED7DNDC102-205} Protein 7 of the Mediator Complex (MED7) with an RBS. This version of MED7 was made to be tagged C-terminally. ---- This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _171_ 0 4745 271 It's complicated false N/A false Jennifer Brophy BBa_J72039_sequence 1 gatctcagctaaaatcaacgtattaaggcactccatagcgatgaaaaaatctaccgaaaacgaatctaccaactaccagtacaaaatccaggaactgcgtaaactgctgaaatctctgctgctgaactacctggaactgatcggtgttctgtctatcaacccggacatgtacgaacgtaaagttgaaaacatccgtaccatcctggttaacatccaccacctgctgaacgaataccgtccgcaccagtctcgtgaatctctgatcatgctgctggaagaacagctggaatacaaacgtggtgaaatccgtgaaatcgaacaggtttgcaaacaggttcacgacaaactgacctctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z