BBa_J72040
1
BBa_J72040
{Bmll6} in BBb
2010-07-14T11:00:00Z
2015-05-08T01:08:26Z
Polymerase Chain Assembly
===Gene Synthesis of Bmll16 {rbs_at_10000.Med21DC1-132>}===
<pre>
Pool mll16 through mll33, assemble by PCA
PCR mll16/mll33 on PCA reaction (457, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll16 {rbs_at_10000.Med21DC1-132>}
----
mll16 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CCATAGAATTCATGAGATCTGAATACCCTACTAAGGACATTC
mll17 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
GTCAGACGGTCGGTCATTTTCTGGCGAATGTCCTTAGTAGGGTAT
mll18 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
AAAATGACCGACCGTCTGACCCAACTCCAAATTTGCCTGGACCAG
mll19 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
GGGTAGCGCAGAACTGTTCGGTCATCTGGTCCAGGCAAATTTGGA
mll20 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CGAACAGTTCTGCGCTACCCTGAACTACATCGACAAAAACCACGG
mll21 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TTCGTTAACGGTCAGACGTTCGAAACCGTGGTTTTTGTCGATGTA
mll22 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
AACGTCTGACCGTTAACGAACCGCAGATGTCTGACAAACACGCTA
mll23 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
GAAAACTCTTCCGGCGGAACAACGGTAGCGTGTTTGTCAGACATC
mll24 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
GTTCCGCCGGAAGAGTTTTCTAACACTATTGACGAACTCTCCACC
mll25 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TCTGACGGGTTTTCAGGATGATGTCGGTGGAGAGTTCGTCAATAG
mll26 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CATCCTGAAAACCCGTCAGATCAACAAACTGATCGACTCTCTGCC
mll27 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TTCCTCTGCGGAAACGTCAACGCCTGGCAGAGAGTCGATCAGTTT
mll28 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TTGACGTTTCCGCAGAGGAACAACTCCGTAAAATCGACATGCTCC
mll29 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
TCTTCAACTTCAACGAGCTTTTTTTGGAGCATGTCGATTTTACGG
mll30 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
AAGCTCGTTGAAGTTGAAGACGAAAAAATCGAAGCTATCAAAAAA
mll31 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CAACGTGACGCAGCAGTTTTTCTTTTTTTTTGATAGCTTCGATTT
mll32 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
AAAACTGCTGCGTCACGTTGACTCTCTGATCGAAGACTTCGTTGA
mll33 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16)
CGTTAGGATCCACCGTCAACGAAGTCTTCGATCAG
</pre>
{rbs_at_10000.Med21DC1-132} Protein 21 of the Mediator Complex (MED21) with an RBS. This version of MED21 was made to be tagged C-terminally.
----
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_171_
0
4745
271
It's complicated
false
N/A
false
Jennifer Brophy
BBa_J72040_sequence
1
gatctgaataccctactaaggacattcgccagaaaatgaccgaccgtctgacccaactccaaatttgcctggaccagatgaccgaacagttctgcgctaccctgaactacatcgacaaaaaccacggtttcgaacgtctgaccgttaacgaaccgcagatgtctgacaaacacgctaccgttgttccgccggaagagttttctaacactattgacgaactctccaccgacatcatcctgaaaacccgtcagatcaacaaactgatcgactctctgccaggcgttgacgtttccgcagaggaacaactccgtaaaatcgacatgctccaaaaaaagctcgttgaagttgaagacgaaaaaatcgaagctatcaaaaaaaaagaaaaactgctgcgtcacgttgactctctgatcgaagacttcgttgacggtg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z