BBa_J72040 1 BBa_J72040 {Bmll6} in BBb 2010-07-14T11:00:00Z 2015-05-08T01:08:26Z Polymerase Chain Assembly ===Gene Synthesis of Bmll16 {rbs_at_10000.Med21DC1-132>}=== <pre> Pool mll16 through mll33, assemble by PCA PCR mll16/mll33 on PCA reaction (457, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll16 {rbs_at_10000.Med21DC1-132>} ---- mll16 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) CCATAGAATTCATGAGATCTGAATACCCTACTAAGGACATTC mll17 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) GTCAGACGGTCGGTCATTTTCTGGCGAATGTCCTTAGTAGGGTAT mll18 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) AAAATGACCGACCGTCTGACCCAACTCCAAATTTGCCTGGACCAG mll19 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) GGGTAGCGCAGAACTGTTCGGTCATCTGGTCCAGGCAAATTTGGA mll20 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) CGAACAGTTCTGCGCTACCCTGAACTACATCGACAAAAACCACGG mll21 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) TTCGTTAACGGTCAGACGTTCGAAACCGTGGTTTTTGTCGATGTA mll22 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) AACGTCTGACCGTTAACGAACCGCAGATGTCTGACAAACACGCTA mll23 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) GAAAACTCTTCCGGCGGAACAACGGTAGCGTGTTTGTCAGACATC mll24 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) GTTCCGCCGGAAGAGTTTTCTAACACTATTGACGAACTCTCCACC mll25 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) TCTGACGGGTTTTCAGGATGATGTCGGTGGAGAGTTCGTCAATAG mll26 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) CATCCTGAAAACCCGTCAGATCAACAAACTGATCGACTCTCTGCC mll27 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) TTCCTCTGCGGAAACGTCAACGCCTGGCAGAGAGTCGATCAGTTT mll28 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) TTGACGTTTCCGCAGAGGAACAACTCCGTAAAATCGACATGCTCC mll29 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) TCTTCAACTTCAACGAGCTTTTTTTGGAGCATGTCGATTTTACGG mll30 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) AAGCTCGTTGAAGTTGAAGACGAAAAAATCGAAGCTATCAAAAAA mll31 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) CAACGTGACGCAGCAGTTTTTCTTTTTTTTTGATAGCTTCGATTT mll32 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) AAAACTGCTGCGTCACGTTGACTCTCTGATCGAAGACTTCGTTGA mll33 PCA assembly of {rbs_at_10000.Med21DC1-132>} (Bmll16) CGTTAGGATCCACCGTCAACGAAGTCTTCGATCAG </pre> {rbs_at_10000.Med21DC1-132} Protein 21 of the Mediator Complex (MED21) with an RBS. This version of MED21 was made to be tagged C-terminally. ---- This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _171_ 0 4745 271 It's complicated false N/A false Jennifer Brophy BBa_J72040_sequence 1 gatctgaataccctactaaggacattcgccagaaaatgaccgaccgtctgacccaactccaaatttgcctggaccagatgaccgaacagttctgcgctaccctgaactacatcgacaaaaaccacggtttcgaacgtctgaccgttaacgaaccgcagatgtctgacaaacacgctaccgttgttccgccggaagagttttctaacactattgacgaactctccaccgacatcatcctgaaaacccgtcagatcaacaaactgatcgactctctgccaggcgttgacgtttccgcagaggaacaactccgtaaaatcgacatgctccaaaaaaagctcgttgaagttgaagacgaaaaaatcgaagctatcaaaaaaaaagaaaaactgctgcgtcacgttgactctctgatcgaagacttcgttgacggtg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z