LOCUS       BBa_J72043              2877 bp    DNA     linear       22-Nov-2024
DEFINITION  pMLLKA 2AB Construction Plasmid
ACCESSION   BBa_J72043
VERSION     BBa_J72043.1  
FEATURES             Location/Qualifiers
     misc_feature    300..1161
                     /label=AmpR
     misc_feature    1458..2273
                     /label=KnR
ORIGIN
        1 gatcctaact cgctcctcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
       61 tgcggcgagc ggtatcagct cactcaaagg cggtaatcaa ttcgacccag ctttcttgta
      121 caaagtggtt gatccttaca gatcccggtt atcggatctc ttagacgtca ggtggcactt
      181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc agaggatcag ttgggtgcac
      421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      541 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      841 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     1021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatcggatct
     1261 ctgaagactc tcgagctgca gtctagggac tcacagctcc tcggatctgc tgatccttca
     1321 actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt
     1381 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     1441 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcc aggccgcgat
     1501 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1561 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
     1621 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     1681 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     1741 ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg
     1801 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     1861 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     1921 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     1981 ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca
     2041 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     2101 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     2161 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     2221 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
     2281 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggag gatctagcag
     2341 ttcaacctgt tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt
     2401 aacgtactaa gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc
     2461 atggctaacg tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa
     2521 attaatataa atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa
     2581 gaatatataa ggcttttaaa gcttttaagg tttaacggtt gtggacaaca agccagggat
     2641 gtaacgcact gagaagccct tagagcctct caaagcaatt ttgagtgaca caggaacact
     2701 taacggctga catgggaatt agccatgggc ccgtgcgaat cacggatctc ccgatcaaga
     2761 attgccatta tcaacaagtt tgtacaaaaa agcaggctcc gaattggtat cacgaggcag
     2821 aatttcagat aaaaaaaatc cttagctttc gctaaggatc tgaagtggaa ttcatga
//