BBa_J72214 1 BBa_J72214 MC1061 upp::pir+FRT-trimethoprimR-FRT 2012-09-16T11:00:00Z 2015-05-08T01:08:29Z n/a MC1061 modified to express pir from plasmid R6K. The sequence is for variant E of an RBS library on pir that resulted in several published variants A-J (PMID 21787416). Strain also known as JTK164. <br> Composition of the insertion: BBa_J72177.rbslib-BBa_J72162.BBa_J72049.BBa_J27001.BBa_J72073.BBa_J72165.BBa_J72166.BBa_J27001 false false _171_ 0 947 95 Not in stock false n/a false Josh Kittleson BBa_J72214_sequence 1 ttattcgataaccacgcgttgttcggaatcagaggctttgatgataccgattttccacgcgttttcaccgttggcattgagcagggcgagggctttgtccacttccggagcaggcagggcaataatcatcccgacgccgcagttgaaggtgcgatacatttcatggtgctcaacgttacctgccgtttgcagccagttgaacacttccggccactgccaggaagattcatcaatcactgcctgagtattatctggcaatacgcgcggaatgttttcccagaagccgccgccggtcaggtgcgcaatggcatgcacatcgaccttttcaatcaactccagcactgacttcacgtaaatgcgggtcggtgccagcagatgatcggctaatggcttaccatcaagttcggtggtttgcggatcacaaccgctgacttcaagaattttgcgcaccagcgaatagccgttcgagtgtggaccgctggaaccgagtgcaatcagcacatcgccgtcgctgactttagagccgtcgatgatttctgatttttctaccacgccaacgcagaaacccgcgacatcgtaatcctcaccgtgatacatccccggcatttctgccgtttcgccacccaccagtgaacagcctgattgcagacaaccttccgcaatgccgctgatcaccgctgaagcggtatcaacatccagttttccggttgcgtaatagtcgaggaaaaacagcggctctgcaccttgcaccaccaggtcattaacgcacatggcgaccagatcaataccaatggtgtcgtgacgttttaagtccattgccagacgcagcttggtacctacgccgtcagtgccagaaaccagcacgggttcacgatatttttgcggcaatgcacacagcgcaccgaagccgcccagaccgcccatcacttccggacgacgcgttttcttcactacgcctttgattcttccaaccagagcattacccgcgtcaatatcaacaccggcatctttgtagctaagagaggttttatcggtcactgcttgggtccccacgcgttacttgcggtagaaaaataaaattcggcgcaattctaacagggaaagcaaacgtttgcgagactgctttacacaacctttttgcacgtcttttccccaggcgcgcggcgaaagaagacttgtgccagggtaaaggttagttttcggatggaataatcttctttcataaccatctgaatataaaataactttatctcaaaccgttatcattttgactaaagtcaacgaaaagaatattgcggatctttgacggctagctcagtcctaggtacagtgctagcggatctcttccggaagactaggtgagactcaaggtcatgatggacgtgaacaaaaaaacgaaaattcgccaccgaaacgagctaaatcacaccctggctcaacttcctttgcccgcaaagcgagtgatgtatatggcgcttgctcccattgatagcaaagaacctcttgaacgagggcgagttttcaaaattagggctgaagaccttgcagcgctcgccaaaatcaccccatcgcttgcttatcgacaattaaaagagggtggtaaattacttggtgccagcaaaatttcgctaagaggggatgatatcattgctttagctaaagagcttaacctgctctttactgctaaaaactcccctgaagagttagaccttaacattattgagtggatagcttattcaaatgatgaaggatacttgtctttaaaattcaccagaaccatagaaccatatatctctagccttattgggaaaaaaaataaattcacaacgcaattgttaacggcaagcttacgcttaagtagccagtattcatcttctctttatcaacttatcaggaagcattactctaattttaagaagaaaaattattttattatttccgttgatgagttaaaggaagagttaatagcttatacttttgataaagatggaaatattgagtacaaataccctgactttcctatttttaaaagggatgtgttaaataaagccattgctgaaattaaaaagaaaacagaaatatcgtttgttggcttcactgttcatgaaaaagaaggaagaaaaattagtaagctgaagttcgaatttgtcgttgatgaagatgaattttctggcgataaagatgatgaagctttttttatgaatttatctgaagctgatgcagcttttctcaaggtatttgatgaaaccgtacctcccaaaaaagctaaggggtgaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggatctgcgaagttcctatactttctagagaataggaacttcggatctttgacagctagctcagtcctaggtataatgctagcggatctttaataaggagaaggaaaagtatgggtcaaagtagcgatgaagccaacgctcccgttgcagggcagtttgcgcttcccctgagtgccacctttggcttaggggatcgcgtacgcaagaaatctggtgccgcttggcagggtcaagtcgtcggttggtattgcacaaaactcactcctgaaggctatgcggtcgagtccgaatcccacccaggctcagtgcaaatttatcctgtggctgcacttgaacgtgtggcctaaggatctctcaacaggcctgctggtaatcgcaggcctttttttttggatctgcgaagttcctatactttctagagaataggaacttcggatctgagtaaagcgcctataacacataatacagaggataatactatgacgcgccgtgctatcggggtgagtgaaagaccgccacttttacagacaatcccgcttagtttgcaacatttgttcgccatgtttggtgcaaccgtcctggtgcccgtcttatttcatattaacccggcgactgtactgttatttaacggtattggaacgctgctgtatctcttcatctgtaaagggaaaattccggcttatcttggttccagctttgcctttatttcaccggtattgttactgttgccgttagggtatgaagtcgcgctgggcggctttattatgtgcggcgtgctgttctgcctggtttcttttatcgtgaagaaagcggggaccggctggctggacgtgctgtttccacctgcggcaatgggcgcaatcgttgccgtcatcggtctggagctggcgggcgtagctgccggtatggcgggtttactcccggctgaagggcaaacgccagactccaaaaccatcatcatctctattaccaccctggcggtcacggttttaggttccgtgctgtttcgtggtttcctggcaattatcccgattttaattggcgtgctggtggggtacgcgctctctttcgcaatgggaattgtcgataccacgccgattattaatgctcactggtttgcgctgccaaccctctatacgccgcgcttcgagtggtttgccattctgactattctgccagcggcgttagtggttattgccgaacacgtagggcacctggtagtaacggctaatatcgtcaaaaaagatctgctgcgcgatccaggtctgcaccgttcgatgtttgctaatggcttgtcgaccgtgatttccggcttctttggctctacgccaaatactacttacggagaaaacattggcgtgatggcgatcacccgtgtttacagtacctgggttatcggcggggcggcgattttcgctatcctgctttcctgcgtcggtaaactggctgccgctatccagatgatcccattgccggtgatgggcggcgtttcgctgctgctttatggtgtcatcggtgcttccggtattcgtgttttgatcgaatcgaaagtggattacaacaaagcacagaacctgatcctgacttccgtgattttgatcatcggcgtcagtggcgcgaaggtaaacatcggcgcggcggagttaaaaggtatggcgctggcgaccatcgtcggtatcggcttaagtttgatcttcaaactgatcagtgtgctgcgccctgaagaagtggtgctggacgcagaagacgcggatataacagacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z