LOCUS BBa_J78002 4125 bp DNA linear 18-Nov-2024 DEFINITION Arg[1] ACCESSION BBa_J78002 VERSION BBa_J78002.1 FEATURES Location/Qualifiers misc_feature 216..1535 /label=Condensation misc_feature 1521..3066 /label=Adenylation misc_feature 3111..3303 /label=Peptidyl-Carrier-Protein CDS 3381..4031 /label=Thioesterase misc_feature 39..56 /label=His tag misc_feature 1..216 /label=pET30 RBS 22..28 /label=RBS misc_feature 4032..4125 /label=pET30 ORIGIN 1 ataattttgt ttaactttaa gaaggagata tacatatgca ccatcatcat catcattctt 61 ctggtctggt gccacgcggt tctggtatga aagaaaccgc tgctgctaaa ttcgaacgcc 121 agcacatgga cagcccagat ctgggtaccg acgacgacga caaggccatg gctgatatcg 181 gatccgaatt cgagctccaa aaatattacc aagtctctca cggtcaaaga aggctttgga 241 ttttgcagca tatcagcgaa tcttctagtg cttataatat taggcttgcc atgagaatta 301 atggcaagtt agatgtttct atattaacag cagcctttca agaactggtg aaccgtcatg 361 aaattctcag aaccactttt accagtgttg gtggcaatat taagcaagtt attcatgagg 421 aaatacccac agaacagtta atcattttta aagatttaag agcagaaaaa gatgctgaac 481 tcttggcaga tgatttaatt caagagtcgg caaatttacc ttttagttta gaaaagttgc 541 cccttattcg ggtcttattg gtgcagatta attcagaaga atttatcttt ggtttgactg 601 tccatcatat tattggggat gcacaatcac ttgatattat ctttcaagag ttctttacat 661 tatactctgc ttatactcaa caaaaaaaag ctgaattacc ccctcttgct ctacagtata 721 aagattatgc ggcctggcaa aatcagtggt tagaaagtga agaagttaga gaacaacatg 781 agtattggtg tgaggttttt tccgaacatc cccctatctt aaacttaccg atagacttcc 841 ctcgacctca agtaaaatct tctcaagcgg cttctcacac ttatcaattt agtcagagtc 901 ttagccaaca attacaaacc tttgccactc aaaataatac gaccctattt attaccctac 961 tgaccttctt taaaatatta ctctaccgcc acaccggcca gcgtgattta gtagtcggta 1021 tccctatttc agcacgaaat catcctgatt tagaaaacca aatcgggttt tatgttaaca 1081 ccttggcttt gagatcttta ttaccagagg gagtaacttt taagcaagtc ttaggggaag 1141 taactaacac ctgtcttgat gcttatgaat atcgtgatta tccttttgat aaattagtat 1201 ccgctctcaa tttagaacgt gatttgtcca gaaatccctt atttgatgtc atgttttctc 1261 tactgagtaa agaaagcaaa actgtcttaa aaattcctgg tttggaacat caaccttatc 1321 ccttaaaacc acggatggct caatttgata tgacttggag cttctttgaa gatagtcata 1381 atcttacatt agtgatcgaa tatgagccag atttattttt gcctgaaacc atcgccagaa 1441 tgaacggtca ctttttacaa attattaaaa gtgtaattca gaatcccgat ggtaaattat 1501 ctgagattaa tttattaacc cctgaagaac agcatcaatt actaatagag tggaatcaaa 1561 ccgaagtcgc ttattctcaa cagtgtctgc accatttatt tgaagaaaaa gttagagata 1621 attccgaggc aatagctctt atttttgagg gcaaaaaatt gacctatcga gagttaaatg 1681 gtcgagctaa tcaagtcggt cactatttac aagaaaaagg cgtaacttca gaagtattag 1741 tggggatttt tattgagcgt tcttttgaga tgatcatcgg aatattggga attatgaaag 1801 ctgggggagc ttatgttcct ttggatccta attatccccc tgagcgcttg gattatatga 1861 tatctgattc ggctatctct ctgttgttaa ctcaacaatc tttagtccaa tttctgccag 1921 aaaatcaagc cgaaatactg tgtttagata cagattggtc aaggatagct aattatagtc 1981 aagagaattt gacctctcca gttaaaacag aaaatttagc ttatgttata tacacttcgg 2041 gttcaacggg aaaacctaaa ggtgtgatga atattcatca aggaatttgc aatactctaa 2101 aatacaatat tgacaattat aatcttaact ctgaagatcg cattctccaa attactccct 2161 ttagttttga tgtttcagtt tgggaagttt tctcatcttt aacatctggt gctaccctag 2221 tcgtgacgaa acccgacggg tataaagata tagattattt aatagattta attgtgcaag 2281 aacaagtaac ttgtttcact tgtgttccct caatattgcg agtttttctg caacatccca 2341 agagcaaaga ttgtcactgt ttaaaacgag taattgtcgg gggagaagcc ttatcttatg 2401 aactcaatca acgatttttt cagcaattaa actgtgaatt atataacgct tatggaccaa 2461 cagaagtagc ggttgatgct actgtctggt gctgtcagcc caattcccag ttaatttcta 2521 ttgggcgccc cattgctaat gtccaagttt atattctcga tagttatctc caaccggttc 2581 ctattggtgt tgctggagaa ttacatattg gtggaatggg tttagcgcgt ggatacctca 2641 accaagctga attaacggct gaaaaattta ttcctcatcc ttttgctgag gggaaattat 2701 ataaaacggg ggatttagct cgctatcttc ctgatggaaa tattgaatat ttagggcgga 2761 ttgataatca agttaagctg agaggtttcc gcattgaatt aggagaaatt cagacagttt 2821 tagaaactca tcccaacgtt gaacaaactg ttgtgattat gcgggaagat accttatata 2881 atcaacggtt agttgcttat gtaatgagaa aagaaccctt attaacttct caggatctgc 2941 gccgtttctt gcagcagaaa ttacccgttt atatgatccc ttctgccttt gtgatgttgt 3001 cggattttcc cttaaatcct aatggtaaaa tagactttca taaattgcct ataccggatg 3061 agacttcttt agttgaatca ccctatttag caccccgcaa tcaaacggaa actatcttag 3121 taagcctttg gcaacagctt ctacaagctg gcaagatagg ggttaatgat aacttctttg 3181 agttgggggg tcattcctta caagctatga atcttatggc tttaatttat gaaaaaatcg 3241 ccatagaaat tcctttatca atgatttatg aaaagccaac agtagccgaa ttaagcgatt 3301 atattatcta tgctcaagag atgaatattc aaccgaaaga acgtccttat gtggtgttta 3361 ataaggagca agaaaaggcc gtctttttat ttccacccgc ccttggtttc gctgctgctt 3421 atgccaattt agcggagtat ataacggatt atgctctcta tacttttcgc tacattagcg 3481 atgaattaat gttagaaaaa tacgctgaat taattgagga tttagctcag gatcaagata 3541 taaaattaat gggacattcc gctggcggat ttttagcgat gttaatggct caacaattag 3601 aaagtcgagg gagagttgtt tctgatgtca ttttactgga tacttacaga ggaggacgtg 3661 aggctaaaaa agctgatatg tcagagattc aagaaggggt tgatgctttc ttattaaatc 3721 ccaaacgtca ggaattaaga ggttactttt tagggaatca aaaactccgc gaccgcactt 3781 atcatcaagt gtgggaatac tttaattttc tttggaattc tgatttaaaa aatgtacaaa 3841 ttcaagggac tattcatttg attcgtgctg agggaaacta tgaagctcga gatgattgga 3901 cacaagcaac aaaaggggaa agaatcaatc attatgccac tggtattcat cgtgaaatga 3961 ttgatcctcc ttatcttccc aaaaacgctc tgattatcaa tagtattctc aacccaaaac 4021 agtcgtaagc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca 4081 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaat //