LOCUS       BBa_J85004              2582 bp    DNA     linear       22-Nov-2024
DEFINITION  3OC6HSL -> non-functional RBS, RFP; constitutive GFP
ACCESSION   BBa_J85004
VERSION     BBa_J85004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..65
                     /label=prefix
     misc_feature    237..237
                     /label=T
     misc_feature    555..555
                     /label=A
     CDS             64..813
                     /label=luxR
     misc_feature    64..819
                     /label=BBa_C0062
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             64..819
                     /label=BBa_C0062
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     misc_feature    46..981
                     /label=BBa_I0462
     misc_feature    990..1009
                     /label=LuxR/HSL
     promoter        1009..1014
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1042..1042
                     /label=start
     misc_feature    990..1044
                     /label=BBa_R0062
     promoter        990..1044
                     /label=BBa_R0062
     misc_feature    1..1044
                     /label=BBa_J09855
     CDS             1069..1749
                     /label=mrfp1
     misc_feature    1069..1749
                     /label=BBa_E1010
     misc_feature    1734..1758
                     /label=barcode
     RBS             1053..1068
                     /label=RBS18.7
     misc_feature    1053..1774
                     /label=BBa_J85002
     misc_feature    1..1774
                     /label=BBa_J85003
     misc_feature    1783..1801
                     /label=TetR 1
     misc_feature    1783..1836
                     /label=BBa_R0040
     promoter        1802..1807
                     /label=-35
     misc_feature    1808..1826
                     /label=TetR 2
     promoter        1825..1830
                     /label=-10
     promoter        1783..1836
                     /label=BBa_R0040
     misc_feature    1849..1852
                     /label=conserved
     RBS             1845..1856
                     /label=BBa_B0034
     CDS             1863..2582
                     /label=GFP protein
     CDS             1863..2582
                     /label=BBa_E0040
     misc_feature    1783..2582
                     /label=BBa_J85202
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag agttactcca gctttagtat ggcttcctcc
     1081 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
     1141 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
     1201 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
     1261 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
     1321 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
     1381 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
     1441 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
     1501 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
     1561 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
     1621 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
     1681 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
     1741 gcttaataac gctgatagtg ctagtgtaga tcgctactag agtccctatc agtgatagag
     1801 attgacatcc ctatcagtga tagagatact gagcactact agagaaagag gagaaatact
     1861 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     1921 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1981 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     2041 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     2101 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     2161 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     2221 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     2281 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     2341 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     2401 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     2461 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     2521 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     2581 aa
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