##gff-version 3
##sequence-region BBa_J85006 1 2636
BBa_J85006	.	engineered_region	1	1828	.	+	0	ID=BBa_J85005;Name=BBa_J85005
BBa_J85006	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_J85005
BBa_J85006	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_J85006	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_J85006	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J85006	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J85006	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J85006	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J85006	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J85006	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J85006	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_J85006	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85006	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85006	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85006	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85006	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_J85006	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85006	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85006	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_J85006	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85006	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_J85006	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J85006	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J85006	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J85006	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J85006	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J85006	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_J85006	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J85006	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J85006	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J85006	.	engineered_region	1053	1774	.	+	0	ID=BBa_J85002;Name=BBa_J85002;Parent=BBa_J85005
BBa_J85006	.	ribosome_entry_site	1053	1068	.	+	0	ID=annotation2124589;Name=RBS18.7;Parent=BBa_J85002
BBa_J85006	.	engineered_region	1069	1749	.	+	0	ID=annotation2124587;Name=BBa_E1010;Parent=BBa_J85002
BBa_J85006	.	CDS	1069	1749	.	+	0	ID=annotation2124586;Name=mrfp1;Parent=BBa_J85002
BBa_J85006	.	engineered_region	1734	1758	.	+	0	ID=annotation2124588;Name=barcode;Parent=BBa_J85002
BBa_J85006	.	terminator	1783	1828	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_J85005
BBa_J85006	.	stem_loop	1795	1817	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J85006	.	engineered_region	1783	1828	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J85006	.	engineered_region	1837	2636	.	+	0	ID=BBa_J85202;Name=BBa_J85202
BBa_J85006	.	ribosome_entry_site	1899	1910	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J85202
BBa_J85006	.	conserved_region	1903	1906	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85006	.	CDS	1917	2636	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J85202
BBa_J85006	.	CDS	1917	2636	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J85006	.	promoter	1837	1890	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J85202
BBa_J85006	.	promoter	1879	1884	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J85006	.	promoter	1856	1861	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J85006	.	engineered_region	1837	1890	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J85006	.	non_covalent_binding_site	1862	1880	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J85006	.	non_covalent_binding_site	1837	1855	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_J85006
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagttactccagctttagtatggcttcctccgaagacgttatcaaagagttcatgcg
tttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaa
ggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttcc
agtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaa
atgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttc
atctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaag
cttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtgg
tcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatc
aaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccg
gtgcttaataacgctgatagtgctagtgtagatcgctactagagagagaatataaaaagccagattattaatccggctt
ttttattattttactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactag
agaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatgg
tgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattt
atttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcga
gatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatt
tttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtta
aaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtataca
tcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaact
agcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaa
tctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacac
atggcatggatgaactatacaaataataa