LOCUS BBa_J85009 836 bp DNA linear 22-Nov-2024 DEFINITION pTet -> aiiA ACCESSION BBa_J85009 VERSION BBa_J85009.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 promoter 1..74 /label=BBa_J13002 CDS 81..836 /label=aiiA CDS 81..836 /label=BBa_C0160 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgacagtaa agaagcttta tttcgtccca gcaggtcgtt 121 gtatgttgga tcattcgtct gttaatagta cattaacacc aggagaatta ttagacttac 181 cggtttggtg ttatcttttg gagactgaag aaggacctat tttagtagat acaggtatgc 241 cagaaagtgc agttaataat gaaggtcttt ttaacggtac atttgtcgaa gggcaggttt 301 taccgaaaat gactgaagaa gatagaatcg tgaatatttt aaaacgggtt ggttatgagc 361 cggaagacct tctttatatt attagttctc acttgcattt tgatcatgca ggaggaaatg 421 gcgcttttat aaatacacca atcattgtac agcgtgctga atatgaggcg gcgcagcata 481 gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa ctacaaaatc attgaaggtg 541 attatgaagt cgtaccagga gttcaattat tgcatacacc aggccatact ccagggcatc 601 aatcgctatt aattgagaca gaaaaatccg gtcctgtatt attaacgatt gatgcatcgt 661 atacgaaaga gaattttgaa aatgaagtgc catttgcggg atttgattca gaattagctt 721 tatcttcaat taaacgttta aaagaagtgg tgatgaaaga gaagccgatt gttttctttg 781 gacatgatat agagcaggaa aggggatgta aagtgttccc tgaatatata taataa //