LOCUS BBa_J85107 2587 bp DNA linear 26-Nov-2024 DEFINITION pLac:RBS:LuxI:pLacIQ:RBS:LuxR:TT:pLux:RBS:RFP ACCESSION BBa_J85107 VERSION BBa_J85107.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..666 /label=LuxI CDS 82..666 /label=BBa_C0161 stem_loop 686..729 /label=stem_loop misc_feature 675..754 /label=BBa_B0010 terminator 675..754 /label=BBa_B0010 stem_loop 770..789 /label=T7 TE misc_feature 796..796 /label=stop polyA_site 790..803 /label=polya misc_feature 763..803 /label=BBa_B0012 terminator 763..803 /label=BBa_B0012 misc_feature 812..848 /label=P(Lac) IQ promoter 814..819 /label=-35 promoter 837..842 /label=-10 promoter 812..848 /label=BBa_I14032 misc_feature 861..864 /label=conserved RBS 857..868 /label=BBa_B0034 misc_feature 875..876 /label=prefix misc_feature 1048..1048 /label=T misc_feature 1366..1366 /label=A CDS 875..1624 /label=luxR misc_feature 875..1630 /label=BBa_C0062 misc_feature 1631..1655 /label=Help:Barcodes CDS 875..1630 /label=BBa_C0062 stem_loop 1675..1718 /label=stem_loop misc_feature 1664..1743 /label=BBa_B0010 terminator 1664..1743 /label=BBa_B0010 stem_loop 1759..1778 /label=T7 TE misc_feature 1785..1785 /label=stop polyA_site 1779..1792 /label=polya misc_feature 1752..1792 /label=BBa_B0012 terminator 1752..1792 /label=BBa_B0012 misc_feature 857..1792 /label=BBa_I0462 misc_feature 1801..1820 /label=LuxR/HSL promoter 1820..1825 /label=-35 promoter 1842..1847 /label=-10 misc_feature 1853..1853 /label=start misc_feature 1801..1855 /label=BBa_R0062 promoter 1801..1855 /label=BBa_R0062 misc_feature 812..1855 /label=BBa_J09855 misc_feature 1868..1871 /label=conserved RBS 1864..1875 /label=BBa_B0034 CDS 1882..2556 /label=mrfp1 misc_feature 2563..2587 /label=Help:Barcodes CDS 1882..2562 /label=BBa_E1010 misc_feature 1864..2587 /label=BBa_I13502 misc_feature 812..2587 /label=BBa_J10000 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 721 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 781 gggtgggcct ttctgcgttt atatactaga gtggtgcaaa acctttcgcg gtatggcatg 841 atagcgccta ctagagaaag aggagaaata ctagatgaaa aacataaatg ccgacgacac 901 atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta atcaatgctt 961 atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca tttatcctca 1021 ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat ggaggcaata 1081 ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta actccaatca 1141 ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat ctccaaatgt 1201 aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta ttcatacggc 1261 taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact atatagatag 1321 tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag ttgataatta 1381 tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga 1441 atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa tattaggttg 1501 cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca atacaacaaa 1561 ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc catactttaa 1621 aaattaataa cactgatagt gctagtgtag atcactacta gagccaggca tcaaataaaa 1681 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 1741 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 1801 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 1861 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt 1921 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt 1981 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg 2041 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt 2101 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg 2161 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg 2221 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt 2281 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa 2341 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac 2401 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac 2461 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag 2521 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt 2581 agatcgc //