##gff-version 3
##sequence-region BBa_J85107 1 2587
BBa_J85107	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J85107	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J85107	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J85107	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J85107	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J85107	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J85107	.	terminator	675	754	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J85107	.	stem_loop	686	729	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85107	.	engineered_region	675	754	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85107	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J85107	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85107	.	engineered_region	812	2587	.	+	0	ID=BBa_J10000;Name=BBa_J10000
BBa_J85107	.	engineered_region	812	1855	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_J10000
BBa_J85107	.	promoter	812	848	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_J85107	.	operator	812	848	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J85107	.	promoter	814	819	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J85107	.	promoter	837	842	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J85107	.	promoter	1801	1855	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_J85107	.	engineered_region	1801	1855	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J85107	.	start_codon	1853	1853	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J85107	.	promoter	1842	1847	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J85107	.	promoter	1820	1825	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J85107	.	non_covalent_binding_site	1801	1820	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J85107	.	engineered_region	857	1792	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_J85107	.	terminator	1752	1792	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_J85107	.	polyA_site	1779	1792	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85107	.	stop_codon	1785	1785	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85107	.	stem_loop	1759	1778	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85107	.	engineered_region	1752	1792	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85107	.	terminator	1664	1743	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_J85107	.	stem_loop	1675	1718	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85107	.	engineered_region	1664	1743	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85107	.	ribosome_entry_site	857	868	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_J85107	.	conserved_region	861	864	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85107	.	CDS	875	1630	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_J85107	.	engineered_tag	1631	1655	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J85107	.	point_mutation	1048	1048	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J85107	.	engineered_region	875	1630	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J85107	.	engineered_region	875	876	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J85107	.	point_mutation	1366	1366	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J85107	.	CDS	875	1624	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J85107	.	engineered_region	1864	2587	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_J10000
BBa_J85107	.	CDS	1882	2562	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_J85107	.	CDS	1882	2556	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J85107	.	engineered_tag	2563	2587	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J85107	.	ribosome_entry_site	1864	1875	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_J85107	.	conserved_region	1868	1871	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85107	.	CDS	82	666	.	+	0	ID=BBa_C0161;Name=BBa_C0161
BBa_J85107	.	CDS	82	666	.	+	0	ID=annotation1891615;Name=LuxI;Parent=BBa_C0161
BBa_J85107	.	terminator	763	803	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J85107	.	polyA_site	790	803	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85107	.	stop_codon	796	796	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85107	.	stem_loop	770	789	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85107	.	engineered_region	763	803	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_J85107
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaat
actagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatat
taatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatg
gttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaat
atgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataa
aaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaac
aatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatga
acataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgattt
aaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgc
agtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttcta
aagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtaca
ggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcttcctccgaa
gacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtg
aaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttg
ggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaa
ctgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggact
cctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgca
gaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatg
cgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgc
cgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacg
tgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc