##gff-version 3
##sequence-region BBa_J85108 1 2732
BBa_J85108	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J85108	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85108	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J85108	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J85108	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J85108	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J85108	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J85108	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J85108	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J85108	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J85108	.	CDS	227	811	.	+	0	ID=BBa_C0161;Name=BBa_C0161
BBa_J85108	.	CDS	227	811	.	+	0	ID=annotation1891615;Name=LuxI;Parent=BBa_C0161
BBa_J85108	.	terminator	908	948	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J85108	.	polyA_site	935	948	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85108	.	stop_codon	941	941	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85108	.	engineered_region	908	948	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85108	.	stem_loop	915	934	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85108	.	terminator	820	899	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J85108	.	stem_loop	831	874	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85108	.	engineered_region	820	899	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85108	.	engineered_region	957	2732	.	+	0	ID=BBa_J10000;Name=BBa_J10000
BBa_J85108	.	engineered_region	957	2000	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_J10000
BBa_J85108	.	promoter	1946	2000	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_J85108	.	non_covalent_binding_site	1946	1965	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J85108	.	promoter	1965	1970	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J85108	.	promoter	1987	1992	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J85108	.	start_codon	1998	1998	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J85108	.	engineered_region	1946	2000	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J85108	.	promoter	957	993	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_J85108	.	promoter	959	964	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J85108	.	operator	957	993	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J85108	.	promoter	982	987	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J85108	.	engineered_region	1002	1937	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_J85108	.	terminator	1809	1888	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_J85108	.	stem_loop	1820	1863	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85108	.	engineered_region	1809	1888	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85108	.	CDS	1020	1775	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_J85108	.	CDS	1020	1769	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J85108	.	engineered_region	1020	1775	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J85108	.	engineered_region	1020	1021	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J85108	.	point_mutation	1193	1193	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J85108	.	engineered_tag	1776	1800	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J85108	.	point_mutation	1511	1511	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J85108	.	terminator	1897	1937	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_J85108	.	polyA_site	1924	1937	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85108	.	stop_codon	1930	1930	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85108	.	engineered_region	1897	1937	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85108	.	stem_loop	1904	1923	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85108	.	ribosome_entry_site	1002	1013	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_J85108	.	conserved_region	1006	1009	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85108	.	engineered_region	2009	2732	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_J10000
BBa_J85108	.	ribosome_entry_site	2009	2020	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_J85108	.	conserved_region	2013	2016	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85108	.	CDS	2027	2707	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_J85108	.	engineered_tag	2708	2732	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J85108	.	CDS	2027	2701	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
>BBa_J85108
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgactataat
gataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaag
caaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatattt
atgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgt
ttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaa
aatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtc
aaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcg
tattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaa
aaagcagtcttaaattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaa
cataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgctta
tctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgata
tttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagt
agattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaat
gtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaa
tgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaat
tgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaa
aaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactg
tcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaac
aggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaaga
aaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaag
agttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcg
tccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcc
ccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccgg
aaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaaga
cggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatg
ggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactga
aagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa
aaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgt
cactccaccggtgcttaataacgctgatagtgctagtgtagatcgc