LOCUS       BBa_J85116              2600 bp    DNA     linear       14-May-2024
DEFINITION  pFTint08
ACCESSION   BBa_J85116
VERSION     BBa_J85116.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=OR3
     misc_feature    11..28
                     /label=OR2
     misc_feature    34..51
                     /label=OR1
     promoter        18..23
                     /label=-35
     promoter        8..13
                     /label=-35
     promoter        30..35
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0053
     promoter        1..54
                     /label=BBa_R0053
     misc_feature    63..82
                     /label=LuxR/HSL
     promoter        177..184
                     /label=-10
     promoter        151..156
                     /label=-35
     misc_feature    190..190
                     /label=start
     misc_feature    192..211
                     /label=Putative LuxR/HSL
     misc_feature    63..213
                     /label=BBa_R0063
     promoter        63..213
                     /label=BBa_R0063
     misc_feature    226..229
                     /label=conserved
     RBS             222..233
                     /label=BBa_B0034
     CDS             240..1115
                     /label=araC
     misc_feature    1116..1148
                     /label=LVA
     misc_feature    1155..1179
                     /label=Help:Barcodes
     CDS             240..1179
                     /label=BBa_C0080
     misc_feature    222..1179
                     /label=BBa_S03550
     misc_feature    1188..1226
                     /label=B1006
     stem_loop       1197..1218
                     /label=modified thr terminator
     polyA_site      1219..1226
                     /label=PolyA
     polyA_site      1188..1196
                     /label=PolyA
     terminator      1188..1226
                     /label=BBa_B1006
     misc_feature    1238..1263
                     /label=c-amp2
     misc_feature    1240..1278
                     /label=araO1
     misc_feature    1277..1306
                     /label=c-amp1
     misc_feature    1307..1335
                     /label=ara1 and ara2
     promoter        1347..1352
                     /label=-35
     promoter        1370..1375
                     /label=-10
     promoter        1235..1383
                     /label=BBa_R0080
     misc_feature    1396..1399
                     /label=conserved
     RBS             1392..1403
                     /label=BBa_B0034
     CDS             1410..2600
                     /label=tetA(C) forward
     primer_bind     1410..1433
                     /label=Fwd primer J31007
     primer_bind     2581..2600
                     /label=Rev primer J31007
     misc_feature    2598..2600
                     /label=stop
     CDS             1410..2600
                     /label=BBa_J31007
ORIGIN
        1 aataaacttg actaaagatt cctttagtag ataatttaag tgttctttaa tttctactag
       61 agacctgtac gatcctacag gtgcttatgt taagtaattg tattcccagc gatacaatag
      121 tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc attaccgttt taatgatata
      181 taacacgcaa aacttgcgac aaacaatagg taatactaga gaaagaggag aaatactaga
      241 tggctgaagc gcaaaatgat cccctgctgc cgggatactc gtttaacgcc catctggtgg
      301 cgggtttaac gccgattgag gccaatggtt atctcgattt ttttatcgac cgaccgctgg
      361 gaatgaaagg ttatattctc aatctcacca ttcgcggtca gggggtggtg aaaaatcagg
      421 gacgagaatt tgtctgccga ccgggtgata ttttgctgtt cccgccagga gagattcatc
      481 actacggtcg tcatccggag gctcgcgaat ggtatcacca gtgggtttac tttcgtccgc
      541 gcgcctactg gcatgaatgg cttaactggc cgtcaatatt tgccaatacg ggtttctttc
      601 gcccggatga agcgcaccag ccgcatttca gcgacctgtt tgggcaaatc attaacgccg
      661 ggcaagggga agggcgctat tcggagctgc tggcgataaa tctgcttgag caattgttac
      721 tgcggcgcat ggaagcgatt aacgagtcgc tccatccacc gatggataat cgggtacgcg
      781 aggcttgtca gtacatcagc gatcacctgg cagacagcaa ttttgatatc gccagcgtcg
      841 cacagcatgt ttgcctgtcg ccgtcgcgtc tgtcacatct tttccgccag cagttaggga
      901 ttagcgtctt aagctggcgc gaggaccaac gcatcagcca ggcgaagctg cttttgagca
      961 ctacccggat gcctatcgcc accgtcggtc gcaatgttgg ttttgacgat caactctatt
     1021 tctcgcgagt atttaaaaaa tgcaccgggg ccagcccgag cgagttccgt gccggttgtg
     1081 aagaaaaagt gaatgatgta gccgtcaagt tgtcagctgc aaacgacgaa aactacgctt
     1141 tagtagctta ataacactga tagtgctagt gtagatcact actagagaaa aaaaaacccc
     1201 gcccctgaca gggcggggtt tttttttact agaggcgtaa caaaagtgtc tataatcacg
     1261 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
     1321 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
     1381 cattactaga gaaagaggag aaatactaga tgaaatctaa caatgcgctc atcgtcatcc
     1441 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
     1501 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc
     1561 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg
     1621 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg
     1681 cgaccacacc cgtcctgtgg atcctctacg ccggacgcat cgtggccggc atcaccggcg
     1741 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     1801 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     1861 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     1921 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac
     1981 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     2041 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2101 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     2161 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggccccgcca
     2221 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     2281 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     2341 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     2401 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg
     2461 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     2521 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     2581 gccgggccac ctcgacctaa
//