##gff-version 3
##sequence-region BBa_J85116 1 2600
BBa_J85116	.	promoter	63	213	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_J85116	.	engineered_region	63	213	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_J85116	.	start_codon	190	190	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_J85116	.	promoter	177	184	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_J85116	.	non_covalent_binding_site	63	82	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_J85116	.	non_covalent_binding_site	192	211	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_J85116	.	promoter	151	156	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_J85116	.	ribosome_entry_site	1392	1403	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J85116	.	conserved_region	1396	1399	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85116	.	engineered_region	222	1179	.	+	0	ID=BBa_S03550;Name=BBa_S03550
BBa_J85116	.	CDS	240	1179	.	+	0	ID=BBa_C0080;Name=BBa_C0080;Parent=BBa_S03550
BBa_J85116	.	engineered_tag	1155	1179	.	+	0	ID=annotation2214004;Name=Help:Barcodes;Parent=BBa_C0080
BBa_J85116	.	tag	1116	1148	.	+	0	ID=annotation308196;Name=LVA;Parent=BBa_C0080
BBa_J85116	.	CDS	240	1115	.	+	0	ID=annotation308191;Name=araC;Parent=BBa_C0080
BBa_J85116	.	ribosome_entry_site	222	233	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03550
BBa_J85116	.	conserved_region	226	229	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85116	.	terminator	1188	1226	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_J85116	.	polyA_site	1188	1196	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_J85116	.	polyA_site	1219	1226	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_J85116	.	engineered_region	1188	1226	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_J85116	.	stem_loop	1197	1218	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_J85116	.	promoter	1	54	.	+	0	ID=BBa_R0053;Name=BBa_R0053
BBa_J85116	.	promoter	18	23	.	+	0	ID=annotation2038;Name=-35;Parent=BBa_R0053
BBa_J85116	.	promoter	8	13	.	+	0	ID=annotation2041;Name=-35;Parent=BBa_R0053
BBa_J85116	.	non_covalent_binding_site	1	3	.	+	0	ID=annotation2035;Name=OR3;Parent=BBa_R0053
BBa_J85116	.	engineered_region	1	54	.	+	0	ID=annotation7069;Name=BBa_R0053;Parent=BBa_R0053
BBa_J85116	.	promoter	30	35	.	+	0	ID=annotation2042;Name=-10;Parent=BBa_R0053
BBa_J85116	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation2037;Name=OR1;Parent=BBa_R0053
BBa_J85116	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation2036;Name=OR2;Parent=BBa_R0053
BBa_J85116	.	promoter	1235	1383	.	+	0	ID=BBa_R0080;Name=BBa_R0080
BBa_J85116	.	non_covalent_binding_site	1238	1263	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_J85116	.	operator	1240	1278	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_J85116	.	non_covalent_binding_site	1277	1306	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_J85116	.	promoter	1370	1375	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_J85116	.	promoter	1347	1352	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_J85116	.	non_covalent_binding_site	1307	1335	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_J85116	.	CDS	1410	2600	.	+	0	ID=BBa_J31007;Name=BBa_J31007
BBa_J85116	.	primer_binding_site	1410	1433	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_J85116	.	primer_binding_site	2581	2600	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_J85116	.	CDS	1410	2600	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_J85116	.	stop_codon	2598	2600	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
>BBa_J85116
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagacctgtacgatcctaca
ggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaat
ccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatact
agatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgat
tgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgc
ggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagaga
ttcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggca
tgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagc
gacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagc
aattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtca
gtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgt
ctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagc
tgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcg
agtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtc
aagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactag
agaaaaaaaaaccccgcccctgacagggcggggtttttttttactagaggcgtaacaaaagtgtctataatcacggcag
aaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcct
acctgacgctttttatcgcaactctctactgtttctccattactagagaaagaggagaaatactagatgaaatctaaca
atgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcct
cttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaattt
ctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagcca
ctatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccgg
cgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatg
agcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccat
tccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcg
tcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcactt
atgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttc
gctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactgg
ccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctg
gcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgc
aggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaac
ttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgta
ggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa