LOCUS BBa_J85118 3443 bp DNA linear 25-Nov-2024 DEFINITION regulatable tetracyclne resistance ACCESSION BBa_J85118 VERSION BBa_J85118.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..729 /label=cII p22 misc_feature 730..762 /label=LVA misc_feature 763..768 /label=stop misc_feature 82..768 /label=BBa_C0053 misc_feature 769..793 /label=Help:Barcodes CDS 82..768 /label=BBa_C0053 misc_feature 802..835 /label=B1002 stem_loop 811..826 /label=stem loop polyA_site 826..832 /label=Poly A tail polyA_site 805..810 /label=Poly A tail terminator 802..835 /label=BBa_B1002 misc_feature 844..846 /label=OR3 misc_feature 854..871 /label=OR2 misc_feature 877..894 /label=OR1 promoter 861..866 /label=-35 promoter 851..856 /label=-35 promoter 873..878 /label=-10 misc_feature 844..897 /label=BBa_R0053 promoter 844..897 /label=BBa_R0053 misc_feature 906..925 /label=LuxR/HSL promoter 1020..1027 /label=-10 promoter 994..999 /label=-35 misc_feature 1033..1033 /label=start misc_feature 1035..1054 /label=Putative LuxR/HSL misc_feature 906..1056 /label=BBa_R0063 promoter 906..1056 /label=BBa_R0063 misc_feature 1069..1072 /label=conserved RBS 1065..1076 /label=BBa_B0034 CDS 1083..1958 /label=araC misc_feature 1959..1991 /label=LVA misc_feature 1998..2022 /label=Help:Barcodes CDS 1083..2022 /label=BBa_C0080 misc_feature 1065..2022 /label=BBa_S03550 misc_feature 2031..2069 /label=B1006 stem_loop 2040..2061 /label=modified thr terminator polyA_site 2062..2069 /label=PolyA polyA_site 2031..2039 /label=PolyA terminator 2031..2069 /label=BBa_B1006 misc_feature 2081..2106 /label=c-amp2 misc_feature 2083..2121 /label=araO1 misc_feature 2120..2149 /label=c-amp1 misc_feature 2150..2178 /label=ara1 and ara2 promoter 2190..2195 /label=-35 promoter 2213..2218 /label=-10 promoter 2078..2226 /label=BBa_R0080 misc_feature 2239..2242 /label=conserved RBS 2235..2246 /label=BBa_B0034 CDS 2253..3443 /label=tetA(C) forward primer_bind 2253..2276 /label=Fwd primer J31007 primer_bind 3424..3443 /label=Rev primer J31007 misc_feature 3441..3443 /label=stop CDS 2253..3443 /label=BBa_J31007 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaataca caattgatgg gtgagcgtat tcgcgctcga 121 agaaaaaaac tcaagattag acaagccgct cttggtaaga tggtgggagt gtctaatgtt 181 gcaatatcgc aatgggagcg ctcggagact gagccaaatg gggagaacct gttggcactt 241 tcgaaggctc ttcagtgctc ccctgactat ttgctgaaag gagatttaag ccagacaaac 301 gttgcctatc atagtaggca tgagccaaga ggatcatacc ctcttatcag ttgggtaagc 361 gcagggcaat ggatggaagc tgtagaacct tatcacaagc gcgcgataga gaactggcac 421 gacaccactg tagattgttc agaagattca ttttggcttg atgtccaagg tgactctatg 481 acagcaccgg cagggttaag cattccagaa ggaatgataa ttctggttga tcccgaagtc 541 gaaccaagaa acggcaagct ggttgttgca aaattagaag gtgaaaacga ggccacattc 601 aaaaaattag ttatggatgc aggccgaaag tttttaaaac cattaaaccc acaatatccg 661 atgatagaaa tcaacggaaa ctgcaaaatc attggcgtag ttgttgacgc aaaactcgca 721 aatcttccag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc 781 tagtgtagat cactactaga gcgcaaaaaa ccccgcttcg gcggggtttt ttcgctacta 841 gagaataaac ttgactaaag attcctttag tagataattt aagtgttctt taatttctac 901 tagagacctg tacgatccta caggtgctta tgttaagtaa ttgtattccc agcgatacaa 961 tagtgtgaca aaaatccaat ttattagaat caaatgtcaa tccattaccg ttttaatgat 1021 atataacacg caaaacttgc gacaaacaat aggtaatact agagaaagag gagaaatact 1081 agatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaac gcccatctgg 1141 tggcgggttt aacgccgatt gaggccaatg gttatctcga tttttttatc gaccgaccgc 1201 tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc 1261 agggacgaga atttgtctgc cgaccgggtg atattttgct gttcccgcca ggagagattc 1321 atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt tactttcgtc 1381 cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat acgggtttct 1441 ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa atcattaacg 1501 ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt gagcaattgt 1561 tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat aatcgggtac 1621 gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat atcgccagcg 1681 tcgcacagca tgtttgcctg tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag 1741 ggattagcgt cttaagctgg cgcgaggacc aacgcatcag ccaggcgaag ctgcttttga 1801 gcactacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac gatcaactct 1861 atttctcgcg agtatttaaa aaatgcaccg gggccagccc gagcgagttc cgtgccggtt 1921 gtgaagaaaa agtgaatgat gtagccgtca agttgtcagc tgcaaacgac gaaaactacg 1981 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag aaaaaaaaac 2041 cccgcccctg acagggcggg gttttttttt actagaggcg taacaaaagt gtctataatc 2101 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat 2161 ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt 2221 ctccattact agagaaagag gagaaatact agatgaaatc taacaatgcg ctcatcgtca 2281 tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg 2341 gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag 2401 cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct 2461 ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca 2521 tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg 2581 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg 2641 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 2701 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca 2761 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc 2821 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga 2881 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg 2941 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc 3001 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggccccg 3061 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg 3121 gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc 3181 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg 3241 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca 3301 ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg 3361 catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat 3421 ggagccgggc cacctcgacc taa //