LOCUS       BBa_J85120              2653 bp    DNA     linear       13-May-2024
DEFINITION  High Population->mRFP (pLac->LuxI; pLacIQ->luxR; pLux->mRFP;)
ACCESSION   BBa_J85120
VERSION     BBa_J85120.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    806..837
                     /label=LVA
     CDS             227..805
                     /label=luxI
     misc_feature    227..844
                     /label=BBa_C0061
     misc_feature    845..869
                     /label=Help:Barcodes
     CDS             227..844
                     /label=BBa_C0061
     misc_feature    209..869
                     /label=BBa_C0261
     misc_feature    1..869
                     /label=BBa_K084014
     misc_feature    878..914
                     /label=P(Lac) IQ
     promoter        880..885
                     /label=-35
     promoter        903..908
                     /label=-10
     promoter        878..914
                     /label=BBa_I14032
     misc_feature    927..930
                     /label=conserved
     RBS             923..934
                     /label=BBa_B0034
     misc_feature    941..942
                     /label=prefix
     misc_feature    1114..1114
                     /label=T
     misc_feature    1432..1432
                     /label=A
     CDS             941..1690
                     /label=luxR
     misc_feature    941..1696
                     /label=BBa_C0062
     misc_feature    1697..1721
                     /label=Help:Barcodes
     CDS             941..1696
                     /label=BBa_C0062
     stem_loop       1741..1784
                     /label=stem_loop
     misc_feature    1730..1809
                     /label=BBa_B0010
     terminator      1730..1809
                     /label=BBa_B0010
     stem_loop       1825..1844
                     /label=T7 TE
     misc_feature    1851..1851
                     /label=stop
     polyA_site      1845..1858
                     /label=polya
     misc_feature    1818..1858
                     /label=BBa_B0012
     terminator      1818..1858
                     /label=BBa_B0012
     misc_feature    923..1858
                     /label=BBa_I0462
     misc_feature    1867..1886
                     /label=LuxR/HSL
     promoter        1886..1891
                     /label=-35
     promoter        1908..1913
                     /label=-10
     misc_feature    1919..1919
                     /label=start
     misc_feature    1867..1921
                     /label=BBa_R0062
     promoter        1867..1921
                     /label=BBa_R0062
     misc_feature    878..1921
                     /label=BBa_J09855
     misc_feature    1934..1937
                     /label=conserved
     RBS             1930..1941
                     /label=BBa_B0034
     CDS             1948..2622
                     /label=mrfp1
     misc_feature    2629..2653
                     /label=Help:Barcodes
     CDS             1948..2628
                     /label=BBa_E1010
     misc_feature    1930..2653
                     /label=BBa_I13502
     misc_feature    878..2653
                     /label=BBa_J10000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat
      241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg
      301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc
      361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt
      421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc
      481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt
      541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact
      601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac
      661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga
      721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga
      781 acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta
      841 ataactctga tagtgctagt gtagatctct actagagtgg tgcaaaacct ttcgcggtat
      901 ggcatgatag cgcctactag agaaagagga gaaatactag atgaaaaaca taaatgccga
      961 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca
     1021 atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta
     1081 tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag
     1141 gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc
     1201 caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc
     1261 aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca
     1321 tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat
     1381 agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga
     1441 taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga
     1501 aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt
     1561 aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac
     1621 aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata
     1681 ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa
     1741 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1801 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1861 ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaataa
     1921 atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc
     1981 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt
     2041 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt
     2101 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct
     2161 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc
     2221 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc
     2281 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc
     2341 gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac
     2401 ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt
     2461 cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt
     2521 gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt
     2581 gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc
     2641 tagtgtagat cgc
//