##gff-version 3 ##sequence-region BBa_J85120 1 2653 BBa_J85120 . engineered_region 878 2653 . + 0 ID=BBa_J10000;Name=BBa_J10000 BBa_J85120 . engineered_region 1930 2653 . + 0 ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_J10000 BBa_J85120 . ribosome_entry_site 1930 1941 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502 BBa_J85120 . conserved_region 1934 1937 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J85120 . CDS 1948 2628 . + 0 ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502 BBa_J85120 . engineered_tag 2629 2653 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_J85120 . CDS 1948 2622 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_J85120 . engineered_region 878 1921 . + 0 ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_J10000 BBa_J85120 . promoter 878 914 . + 0 ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855 BBa_J85120 . promoter 903 908 . + 0 ID=annotation1028344;Name=-10;Parent=BBa_I14032 BBa_J85120 . operator 878 914 . + 0 ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032 BBa_J85120 . promoter 880 885 . + 0 ID=annotation1028343;Name=-35;Parent=BBa_I14032 BBa_J85120 . engineered_region 923 1858 . + 0 ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855 BBa_J85120 . terminator 1818 1858 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462 BBa_J85120 . engineered_region 1818 1858 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J85120 . stem_loop 1825 1844 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J85120 . polyA_site 1845 1858 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J85120 . stop_codon 1851 1851 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J85120 . CDS 941 1696 . + 0 ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462 BBa_J85120 . engineered_tag 1697 1721 . + 0 ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062 BBa_J85120 . engineered_region 941 942 . + 0 ID=annotation1762;Name=prefix;Parent=BBa_C0062 BBa_J85120 . point_mutation 1432 1432 . + 0 ID=annotation1765;Name=A;Parent=BBa_C0062 BBa_J85120 . CDS 941 1690 . + 0 ID=annotation1766;Name=luxR;Parent=BBa_C0062 BBa_J85120 . point_mutation 1114 1114 . + 0 ID=annotation1764;Name=T;Parent=BBa_C0062 BBa_J85120 . engineered_region 941 1696 . + 0 ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062 BBa_J85120 . terminator 1730 1809 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462 BBa_J85120 . engineered_region 1730 1809 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J85120 . stem_loop 1741 1784 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J85120 . ribosome_entry_site 923 934 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462 BBa_J85120 . conserved_region 927 930 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J85120 . promoter 1867 1921 . + 0 ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855 BBa_J85120 . promoter 1886 1891 . + 0 ID=annotation2046;Name=-35;Parent=BBa_R0062 BBa_J85120 . promoter 1908 1913 . + 0 ID=annotation2047;Name=-10;Parent=BBa_R0062 BBa_J85120 . start_codon 1919 1919 . + 0 ID=annotation2048;Name=start;Parent=BBa_R0062 BBa_J85120 . engineered_region 1867 1921 . + 0 ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062 BBa_J85120 . non_covalent_binding_site 1867 1886 . + 0 ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062 BBa_J85120 . engineered_region 1 869 . + 0 ID=BBa_K084014;Name=BBa_K084014 BBa_J85120 . engineered_region 209 869 . + 0 ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K084014 BBa_J85120 . CDS 227 844 . + 0 ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261 BBa_J85120 . CDS 227 805 . + 0 ID=annotation1761;Name=luxI;Parent=BBa_C0061 BBa_J85120 . tag 806 837 . + 0 ID=annotation1760;Name=LVA;Parent=BBa_C0061 BBa_J85120 . engineered_tag 845 869 . + 0 ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061 BBa_J85120 . engineered_region 227 844 . + 0 ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061 BBa_J85120 . ribosome_entry_site 209 220 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261 BBa_J85120 . conserved_region 213 216 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J85120 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K084014 BBa_J85120 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_J85120 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_J85120 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_J85120 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_J85120 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_J85120 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_J85120 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 >BBa_J85120 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgactataat gataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaag caaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatattt atgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgt ttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaa aatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtc aaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcg tattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaa aaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc tactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaa cataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgctta tctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgata tttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagt agattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaat gtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaa tgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaat tgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaa aaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactg tcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaac aggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatc aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaaga aaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaag agttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcg tccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcc ccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccgg aaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaaga cggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatg ggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactga aagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa aaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgt cactccaccggtgcttaataacgctgatagtgctagtgtagatcgc