LOCUS BBa_J85121 6286 bp DNA linear 22-Nov-2024 DEFINITION error detection and correction - tetr. resistance loss ACCESSION BBa_J85121 VERSION BBa_J85121.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..811 /label=LuxI CDS 227..811 /label=BBa_C0161 stem_loop 831..874 /label=stem_loop misc_feature 820..899 /label=BBa_B0010 terminator 820..899 /label=BBa_B0010 stem_loop 915..934 /label=T7 TE misc_feature 941..941 /label=stop polyA_site 935..948 /label=polya misc_feature 908..948 /label=BBa_B0012 terminator 908..948 /label=BBa_B0012 misc_feature 957..993 /label=P(Lac) IQ promoter 959..964 /label=-35 promoter 982..987 /label=-10 promoter 957..993 /label=BBa_I14032 misc_feature 1006..1009 /label=conserved RBS 1002..1013 /label=BBa_B0034 misc_feature 1020..1021 /label=prefix misc_feature 1193..1193 /label=T misc_feature 1511..1511 /label=A CDS 1020..1769 /label=luxR misc_feature 1020..1775 /label=BBa_C0062 misc_feature 1776..1800 /label=Help:Barcodes CDS 1020..1775 /label=BBa_C0062 stem_loop 1820..1863 /label=stem_loop misc_feature 1809..1888 /label=BBa_B0010 terminator 1809..1888 /label=BBa_B0010 stem_loop 1904..1923 /label=T7 TE misc_feature 1930..1930 /label=stop polyA_site 1924..1937 /label=polya misc_feature 1897..1937 /label=BBa_B0012 terminator 1897..1937 /label=BBa_B0012 misc_feature 1002..1937 /label=BBa_I0462 misc_feature 1946..1965 /label=LuxR/HSL promoter 1965..1970 /label=-35 promoter 1987..1992 /label=-10 misc_feature 1998..1998 /label=start misc_feature 1946..2000 /label=BBa_R0062 promoter 1946..2000 /label=BBa_R0062 misc_feature 957..2000 /label=BBa_J09855 misc_feature 2013..2016 /label=conserved RBS 2009..2020 /label=BBa_B0034 CDS 2027..2701 /label=mrfp1 misc_feature 2708..2732 /label=Help:Barcodes CDS 2027..2707 /label=BBa_E1010 misc_feature 2009..2732 /label=BBa_I13502 misc_feature 957..2732 /label=BBa_J10000 CDS 2741..2828 /label=end of LacI coding region (inactive) misc_feature 2741..2940 /label=BBa_R0010 misc_feature 2829..2866 /label=CAP binding site promoter 2877..2882 /label=-35 promoter 2901..2906 /label=-10 misc_feature 2906..2940 /label=LacI binding site misc_feature 2913..2913 /label=start promoter 2741..2940 /label=BBa_R0010 misc_feature 2953..2956 /label=conserved RBS 2949..2960 /label=BBa_B0034 CDS 2967..3614 /label=cII p22 misc_feature 3615..3647 /label=LVA misc_feature 3648..3653 /label=stop misc_feature 2967..3653 /label=BBa_C0053 misc_feature 3654..3678 /label=Help:Barcodes CDS 2967..3653 /label=BBa_C0053 misc_feature 3687..3689 /label=OR3 misc_feature 3697..3714 /label=OR2 misc_feature 3720..3737 /label=OR1 promoter 3704..3709 /label=-35 promoter 3694..3699 /label=-35 promoter 3716..3721 /label=-10 misc_feature 3687..3740 /label=BBa_R0053 promoter 3687..3740 /label=BBa_R0053 misc_feature 3749..3768 /label=LuxR/HSL promoter 3863..3870 /label=-10 promoter 3837..3842 /label=-35 misc_feature 3876..3876 /label=start misc_feature 3878..3897 /label=Putative LuxR/HSL misc_feature 3749..3899 /label=BBa_R0063 promoter 3749..3899 /label=BBa_R0063 misc_feature 3912..3915 /label=conserved RBS 3908..3919 /label=BBa_B0034 CDS 3926..4801 /label=araC misc_feature 4802..4834 /label=LVA misc_feature 4841..4865 /label=Help:Barcodes CDS 3926..4865 /label=BBa_C0080 misc_feature 3908..4865 /label=BBa_S03550 misc_feature 4874..4912 /label=B1006 stem_loop 4883..4904 /label=modified thr terminator polyA_site 4905..4912 /label=PolyA polyA_site 4874..4882 /label=PolyA terminator 4874..4912 /label=BBa_B1006 misc_feature 4924..4949 /label=c-amp2 misc_feature 4926..4964 /label=araO1 misc_feature 4963..4992 /label=c-amp1 misc_feature 4993..5021 /label=ara1 and ara2 promoter 5033..5038 /label=-35 promoter 5056..5061 /label=-10 promoter 4921..5069 /label=BBa_R0080 misc_feature 5082..5085 /label=conserved RBS 5078..5089 /label=BBa_B0034 CDS 5096..6286 /label=tetA(C) forward primer_bind 5096..5119 /label=Fwd primer J31007 primer_bind 6267..6286 /label=Rev primer J31007 misc_feature 6284..6286 /label=stop CDS 5096..6286 /label=BBa_J31007 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat 241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg 301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc 361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt 421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc 481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt 541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact 601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac 661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga 721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga 781 acagtttaaa aaagcagtct taaattaata atactagagc caggcatcaa ataaaacgaa 841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtggt 961 gcaaaacctt tcgcggtatg gcatgatagc gcctactaga gaaagaggag aaatactaga 1021 tgaaaaacat aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa 1081 gcaataatga tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt 1141 atttactcgc gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata 1201 attaccctaa aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta 1261 tagtagatta ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg 1321 ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg 1381 ggtttagttt ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt 1441 cagaaaaaga caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa 1501 ttgttccttc tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca 1561 acgatttaac caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt 1621 gggatatttc aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg 1681 cgcaaatgaa actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag 1741 gagcaattga ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac 1801 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1861 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1921 gcctttctgc gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg 1981 gtttgttata gtcgaataaa tactagagaa agaggagaaa tactagatgg cttcctccga 2041 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 2101 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 2161 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 2221 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 2281 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 2341 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 2401 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 2461 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 2521 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 2581 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 2641 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 2701 ttaataacgc tgatagtgct agtgtagatc gctactagag caatacgcaa accgcctctc 2761 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 2821 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 2881 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 2941 tactagagaa agaggagaaa tactagatga atacacaatt gatgggtgag cgtattcgcg 3001 ctcgaagaaa aaaactcaag attagacaag ccgctcttgg taagatggtg ggagtgtcta 3061 atgttgcaat atcgcaatgg gagcgctcgg agactgagcc aaatggggag aacctgttgg 3121 cactttcgaa ggctcttcag tgctcccctg actatttgct gaaaggagat ttaagccaga 3181 caaacgttgc ctatcatagt aggcatgagc caagaggatc ataccctctt atcagttggg 3241 taagcgcagg gcaatggatg gaagctgtag aaccttatca caagcgcgcg atagagaact 3301 ggcacgacac cactgtagat tgttcagaag attcattttg gcttgatgtc caaggtgact 3361 ctatgacagc accggcaggg ttaagcattc cagaaggaat gataattctg gttgatcccg 3421 aagtcgaacc aagaaacggc aagctggttg ttgcaaaatt agaaggtgaa aacgaggcca 3481 cattcaaaaa attagttatg gatgcaggcc gaaagttttt aaaaccatta aacccacaat 3541 atccgatgat agaaatcaac ggaaactgca aaatcattgg cgtagttgtt gacgcaaaac 3601 tcgcaaatct tccagctgca aacgacgaaa actacgcttt agtagcttaa taacactgat 3661 agtgctagtg tagatcacta ctagagaata aacttgacta aagattcctt tagtagataa 3721 tttaagtgtt ctttaatttc tactagagac ctgtacgatc ctacaggtgc ttatgttaag 3781 taattgtatt cccagcgata caatagtgtg acaaaaatcc aatttattag aatcaaatgt 3841 caatccatta ccgttttaat gatatataac acgcaaaact tgcgacaaac aataggtaat 3901 actagagaaa gaggagaaat actagatggc tgaagcgcaa aatgatcccc tgctgccggg 3961 atactcgttt aacgcccatc tggtggcggg tttaacgccg attgaggcca atggttatct 4021 cgattttttt atcgaccgac cgctgggaat gaaaggttat attctcaatc tcaccattcg 4081 cggtcagggg gtggtgaaaa atcagggacg agaatttgtc tgccgaccgg gtgatatttt 4141 gctgttcccg ccaggagaga ttcatcacta cggtcgtcat ccggaggctc gcgaatggta 4201 tcaccagtgg gtttactttc gtccgcgcgc ctactggcat gaatggctta actggccgtc 4261 aatatttgcc aatacgggtt tctttcgccc ggatgaagcg caccagccgc atttcagcga 4321 cctgtttggg caaatcatta acgccgggca aggggaaggg cgctattcgg agctgctggc 4381 gataaatctg cttgagcaat tgttactgcg gcgcatggaa gcgattaacg agtcgctcca 4441 tccaccgatg gataatcggg tacgcgaggc ttgtcagtac atcagcgatc acctggcaga 4501 cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc ctgtcgccgt cgcgtctgtc 4561 acatcttttc cgccagcagt tagggattag cgtcttaagc tggcgcgagg accaacgcat 4621 cagccaggcg aagctgcttt tgagcactac ccggatgcct atcgccaccg tcggtcgcaa 4681 tgttggtttt gacgatcaac tctatttctc gcgagtattt aaaaaatgca ccggggccag 4741 cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat gatgtagccg tcaagttgtc 4801 agctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag 4861 atcactacta gagaaaaaaa aaccccgccc ctgacagggc ggggtttttt tttactagag 4921 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt 4981 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt 5041 tttatcgcaa ctctctactg tttctccatt actagagaaa gaggagaaat actagatgaa 5101 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg 5161 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc 5221 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt 5281 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg 5341 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg 5401 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga 5461 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt 5521 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc 5581 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca 5641 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc 5701 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 5761 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg 5821 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc 5881 tcaagccttc gtcactggcc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc 5941 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat 6001 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc 6061 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc 6121 tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt atgccgcctc 6181 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct 6241 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctaa //