##gff-version 3
##sequence-region BBa_J85127 1 1414
BBa_J85127	.	promoter	1361	1414	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J85127	.	promoter	1380	1385	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J85127	.	promoter	1403	1408	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J85127	.	operator	1363	1379	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J85127	.	engineered_region	1361	1414	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J85127	.	operator	1386	1402	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J85127	.	engineered_region	46	1352	.	+	0	ID=BBa_P0312;Name=BBa_P0312
BBa_J85127	.	terminator	1312	1352	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0312
BBa_J85127	.	polyA_site	1339	1352	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85127	.	stop_codon	1345	1345	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85127	.	stem_loop	1319	1338	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85127	.	engineered_region	1312	1352	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85127	.	terminator	1224	1303	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0312
BBa_J85127	.	stem_loop	1235	1278	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85127	.	engineered_region	1224	1303	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85127	.	CDS	63	1190	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0312
BBa_J85127	.	engineered_region	63	1190	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J85127	.	engineered_tag	1191	1215	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J85127	.	CDS	63	1190	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J85127	.	tag	1152	1190	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J85127	.	ribosome_entry_site	46	56	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0312
BBa_J85127	.	engineered_region	46	56	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J85127	.	ribosome_entry_site	46	56	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J85127	.	ribosome_entry_site	52	55	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J85127	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_J85127	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J85127	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J85127	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
>BBa_J85127
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagtcacacaggactactagatggtgaatgtgaaacc
agtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccac
gtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaac
tggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggc
gattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaa
gcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattg
ctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattatttt
ctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggc
ccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatag
cggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccac
tgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcg
gatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatt
ttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgtt
gcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattca
ttaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctt
aataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca