LOCUS       BBa_J85128               809 bp    DNA     linear       29-Apr-2024
DEFINITION  aTc inducible plasmid
ACCESSION   BBa_J85128
VERSION     BBa_J85128.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     RBS             46..56
                     /label=RBS-4\Weaker
     RBS             52..55
                     /label=RBS
     misc_feature    46..56
                     /label=BBa_B0033
     RBS             46..56
                     /label=BBa_B0033
     CDS             66..682
                     /label=tetR
     misc_feature    683..716
                     /label=SsrA
     misc_feature    723..747
                     /label=Help:Barcodes
     CDS             63..722
                     /label=BBa_C0040
     misc_feature    46..747
                     /label=BBa_S0107
     misc_feature    756..774
                     /label=TetR 1
     misc_feature    756..809
                     /label=BBa_R0040
     promoter        775..780
                     /label=-35
     misc_feature    781..799
                     /label=TetR 2
     promoter        798..803
                     /label=-10
     promoter        756..809
                     /label=BBa_R0040
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagtcaca caggactact
       61 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg
      121 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta
      181 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt
      241 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac
      301 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac
      361 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt
      421 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt
      481 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa
      541 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc
      601 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac
      661 aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      721 aacactgata gtgctagtgt agatcactac tagagtccct atcagtgata gagattgaca
      781 tccctatcag tgatagagat actgagcac
//