##gff-version 3
##sequence-region BBa_J85128 1 809
BBa_J85128	.	engineered_region	46	747	.	+	0	ID=BBa_S0107;Name=BBa_S0107
BBa_J85128	.	ribosome_entry_site	46	56	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_J85128	.	ribosome_entry_site	46	56	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_J85128	.	ribosome_entry_site	52	55	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_J85128	.	engineered_region	46	56	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_J85128	.	CDS	63	722	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_J85128	.	engineered_tag	723	747	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J85128	.	CDS	66	682	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J85128	.	tag	683	716	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J85128	.	promoter	756	809	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J85128	.	non_covalent_binding_site	756	774	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J85128	.	engineered_region	756	809	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J85128	.	promoter	798	803	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J85128	.	promoter	775	780	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J85128	.	non_covalent_binding_site	781	799	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J85128	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_J85128	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J85128	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J85128	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
>BBa_J85128
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagtcacacaggactactagatgtccagattagataa
aagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccag
aagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgaga
tgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaag
ttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaa
actctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtgg
ggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactga
tagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcctt
gaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttag
tagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcac