##gff-version 3
##sequence-region BBa_J85139 1 1756
BBa_J85139	.	engineered_region	63	994	.	+	0	ID=BBa_P0151;Name=BBa_P0151
BBa_J85139	.	ribosome_entry_site	63	76	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0151
BBa_J85139	.	conserved_region	69	72	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J85139	.	CDS	83	832	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0151
BBa_J85139	.	tag	794	826	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J85139	.	engineered_tag	833	857	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J85139	.	CDS	86	793	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J85139	.	terminator	954	994	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0151
BBa_J85139	.	stem_loop	961	980	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85139	.	stop_codon	987	987	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85139	.	polyA_site	981	994	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85139	.	engineered_region	954	994	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85139	.	terminator	866	945	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0151
BBa_J85139	.	stem_loop	877	920	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85139	.	engineered_region	866	945	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85139	.	promoter	1003	1070	.	+	0	ID=BBa_K415032;Name=BBa_K415032
BBa_J85139	.	operator	1003	1023	.	+	0	ID=annotation2169674;Name=LuxR-AHL Binding Box;Parent=BBa_K415032
BBa_J85139	.	operator	1054	1070	.	+	0	ID=annotation2169676;Name=Lambda Repressor OR1;Parent=BBa_K415032
BBa_J85139	.	start_codon	1053	1053	.	+	0	ID=annotation2169677;Name=Transcription Start Site;Parent=BBa_K415032
BBa_J85139	.	non_covalent_binding_site	1044	1049	.	+	0	ID=annotation2169675;Name=Pseudo-Pribnow Box;Parent=BBa_K415032
BBa_J85139	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J85139	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J85139	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J85139	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J85139	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J85139	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J85139	.	engineered_region	1079	1756	.	+	0	ID=BBa_J85101;Name=BBa_J85101
BBa_J85139	.	CDS	1097	1756	.	+	0	ID=BBa_J31005;Name=BBa_J31005;Parent=BBa_J85101
BBa_J85139	.	sequence_feature	1097	1756	.	+	0	ID=annotation1884999;Name=CmR gene;Parent=BBa_J31005
BBa_J85139	.	ribosome_entry_site	1079	1090	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J85101
BBa_J85139	.	conserved_region	1083	1086	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J85139
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaacctac
tagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaa
agaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatt
taatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagc
ccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtacc
ctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggt
aagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcc
aagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagcca
gacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccaca
gtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgttt
ggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacg
caagaaaatggtttgttatagtcgaatacctctggcggtgatatactagagaaagaggagaaatactagatggagaaaa
aaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctca
atgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttat
ccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctgg
tgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaata
ccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccct
aaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggcca
atatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggc
gattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgag
tggcagggcggggcgtaa