BBa_J85139
type: ComponentDefinition
persistent identity: BBa J85139
display id: BBa_J85139
version: 1
was generated by: iGEM to SBOL conversion
description: error correction part 2
created: 2011-02-14T12:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: basic BBricks
owned by: james
owned by: myers
mutable description: error correction part 2
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 4459
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: basic biobricks assembly
star: false
creator: Cristian Grecu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J85101
component: BBa_K415032
component: BBa_P0151
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_P0151
sequence annotation: BBa_K415032
sequence annotation: BBa_J85101
sequence: https://synbiohub.org/public/igem/BBa_J85139_sequence/1

BBa_J85101
type: Component
persistent identity: component2115551
display id: component2115551
version: 1
top level: BBa_J85139
definition: BBa_J85101
access: public

BBa_K415032
type: Component
persistent identity: component2115546
display id: component2115546
version: 1
top level: BBa_J85139
definition: BBa_K415032
access: public

BBa_P0151
type: Component
persistent identity: component2115545
display id: component2115545
version: 1
top level: BBa_J85139
definition: BBa_P0151
access: public

BBa_R0040
type: Component
persistent identity: component2115528
display id: component2115528
version: 1
top level: BBa_J85139
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2115528
display id: annotation2115528
version: 1
top level: BBa_J85139
location: https://synbiohub.org/public/igem/BBa_J85139/annotation2115528/range2115528/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J85139/annotation2115528/range2115528/1
type: Range
persistent identity: range2115528
display id: range2115528
version: 1
top level: BBa_J85139
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_P0151
type: SequenceAnnotation
persistent identity: annotation2115545
display id: annotation2115545
version: 1
top level: BBa_J85139
location: https://synbiohub.org/public/igem/BBa_J85139/annotation2115545/range2115545/1
component: BBa_P0151

https://synbiohub.org/public/igem/BBa_J85139/annotation2115545/range2115545/1
type: Range
persistent identity: range2115545
display id: range2115545
version: 1
top level: BBa_J85139
direction: direction/reverse
start: 63
end: 994
orientation: inline

BBa_K415032
type: SequenceAnnotation
persistent identity: annotation2115546
display id: annotation2115546
version: 1
top level: BBa_J85139
location: https://synbiohub.org/public/igem/BBa_J85139/annotation2115546/range2115546/1
component: BBa_K415032

https://synbiohub.org/public/igem/BBa_J85139/annotation2115546/range2115546/1
type: Range
persistent identity: range2115546
display id: range2115546
version: 1
top level: BBa_J85139
direction: direction/reverse
start: 1003
end: 1070
orientation: inline

BBa_J85101
type: SequenceAnnotation
persistent identity: annotation2115551
display id: annotation2115551
version: 1
top level: BBa_J85139
location: https://synbiohub.org/public/igem/BBa_J85139/annotation2115551/range2115551/1
component: BBa_J85101

https://synbiohub.org/public/igem/BBa_J85139/annotation2115551/range2115551/1
type: Range
persistent identity: range2115551
display id: range2115551
version: 1
top level: BBa_J85139
direction: direction/reverse
start: 1079
end: 1756
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_P0151
type: ComponentDefinition
persistent identity: BBa P0151
display id: BBa_P0151
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS -> cI (lambda) [S0165]
created: 2004-04-24T11:00:00Z
modified: 2015-05-08T01:14:10Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Protein generator converting TIPS to the protein cI from lambda. Used as the input section for Quad Part Inverters Q01150 and Q01151.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_C0051
component: BBa_B0031
sequence annotation: BBa_B0031
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0151_sequence/1

BBa_B0012
type: Component
persistent identity: component944529
display id: component944529
version: 1
top level: BBa_P0151
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component944519
display id: component944519
version: 1
top level: BBa_P0151
definition: BBa_B0010
access: public

BBa_C0051
type: Component
persistent identity: component944513
display id: component944513
version: 1
top level: BBa_P0151
definition: cI lam
access: public

BBa_B0031
type: Component
persistent identity: component944503
display id: component944503
version: 1
top level: BBa_P0151
definition: BBa_B0031
access: public

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation944503
display id: annotation944503
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_P0151/annotation944503/range944503/1
type: Range
persistent identity: range944503
display id: range944503
version: 1
top level: BBa_P0151
direction: direction/forward
start: 1
end: 14
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation944513
display id: annotation944513
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_P0151/annotation944513/range944513/1
type: Range
persistent identity: range944513
display id: range944513
version: 1
top level: BBa_P0151
direction: direction/forward
start: 21
end: 770
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944519
display id: annotation944519
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0151/annotation944519/range944519/1
type: Range
persistent identity: range944519
display id: range944519
version: 1
top level: BBa_P0151
direction: direction/forward
start: 804
end: 883
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944529
display id: annotation944529
version: 1
top level: BBa_P0151
location: https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0151/annotation944529/range944529/1
type: Range
persistent identity: range944529
display id: range944529
version: 1
top level: BBa_P0151
direction: direction/forward
start: 892
end: 932
orientation: inline

BBa_J85101
type: ComponentDefinition
persistent identity: BBa J85101
display id: BBa_J85101
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-CmR
created: 2010-01-06T12:00:00Z
modified: 2015-05-08T01:08:29Z
mutable provenance: made of BBa biobricks
owned by: james
owned by: myers
mutable description: intermediate part to be used for inducible chloramphenicol resistance
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 4459
owning group id: 406
sample status: In stock
status: status/Available
bookmark: false
mutable notes: ---
star: false
creator: Cristian Grecu
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J31005
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_J31005
sequence: https://synbiohub.org/public/igem/BBa_J85101_sequence/1

BBa_J31005
type: Component
persistent identity: component2065240
display id: component2065240
version: 1
top level: BBa_J85101
definition: CmR
access: public

BBa_B0034
type: Component
persistent identity: component2065238
display id: component2065238
version: 1
top level: BBa_J85101
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2065238
display id: annotation2065238
version: 1
top level: BBa_J85101
location: https://synbiohub.org/public/igem/BBa_J85101/annotation2065238/range2065238/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J85101/annotation2065238/range2065238/1
type: Range
persistent identity: range2065238
display id: range2065238
version: 1
top level: BBa_J85101
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J31005
type: SequenceAnnotation
persistent identity: annotation2065240
display id: annotation2065240
version: 1
top level: BBa_J85101
location: https://synbiohub.org/public/igem/BBa_J85101/annotation2065240/range2065240/1
component: BBa_J31005

https://synbiohub.org/public/igem/BBa_J85101/annotation2065240/range2065240/1
type: Range
persistent identity: range2065240
display id: range2065240
version: 1
top level: BBa_J85101
direction: direction/reverse
start: 19
end: 678
orientation: inline

BBa_K415032
type: ComponentDefinition
persistent identity: BBa K415032
display id: BBa_K415032
version: 1
was generated by: iGEM to SBOL conversion
description: Improved PLux/cI-OR Promoter
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:26Z
mutable provenance: Spatiotemporal Control of Gene Expression with Pulse-Generating Networks Author(s): Subhayu Basu, Rishabh Mehreja, Stephan Thiberge, Ming-Tang Chen, Ron Weiss, Lonnie O'Neal Ingram Source: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 17 (Apr. 27, 2004), pp. 6355-6360
owned by: james
owned by: myers
mutable description: This part is a promoter that was designed as a correction to the anomalous BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.
discontinued: false
dominant: false
group u list: _525_
m user id: 0
owner id: 7331
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: SDM performed on sequence.
star: false
creator: Paul Muir
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR-AHL Binding Box
sequence annotation: Pseudo-Pribnow Box
sequence annotation: Transcription Start Site
sequence annotation: Lambda Repressor OR1
sequence: https://synbiohub.org/public/igem/BBa_K415032_sequence/1

LuxR-AHL Binding Box
type: SequenceAnnotation
persistent identity: annotation2169674
display id: annotation2169674
version: 1
top level: BBa_K415032
location: https://synbiohub.org/public/igem/BBa_K415032/annotation2169674/range2169674/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K415032/annotation2169674/range2169674/1
type: Range
persistent identity: range2169674
display id: range2169674
version: 1
top level: BBa_K415032
direction: direction/forward
start: 1
end: 21
orientation: inline

Pseudo-Pribnow Box
type: SequenceAnnotation
persistent identity: annotation2169675
display id: annotation2169675
version: 1
top level: BBa_K415032
location: https://synbiohub.org/public/igem/BBa_K415032/annotation2169675/range2169675/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K415032/annotation2169675/range2169675/1
type: Range
persistent identity: range2169675
display id: range2169675
version: 1
top level: BBa_K415032
direction: direction/forward
start: 42
end: 47
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation2169677
display id: annotation2169677
version: 1
top level: BBa_K415032
location: https://synbiohub.org/public/igem/BBa_K415032/annotation2169677/range2169677/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K415032/annotation2169677/range2169677/1
type: Range
persistent identity: range2169677
display id: range2169677
version: 1
top level: BBa_K415032
direction: direction/forward
start: 51
end: 51
orientation: inline

Lambda Repressor OR1
type: SequenceAnnotation
persistent identity: annotation2169676
display id: annotation2169676
version: 1
top level: BBa_K415032
location: https://synbiohub.org/public/igem/BBa_K415032/annotation2169676/range2169676/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K415032/annotation2169676/range2169676/1
type: Range
persistent identity: range2169676
display id: range2169676
version: 1
top level: BBa_K415032
direction: direction/forward
start: 52
end: 68
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

CmR
type: ComponentDefinition
persistent identity: BBa J31005
display id: BBa_J31005
version: 1
was generated by: iGEM to SBOL conversion
description: chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: pSB1AC3
owned by: james
owned by: myers
mutable description: When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
discontinued: false
dominant: true
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is cloned into pSB1A2.
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: CmR gene
sequence: https://synbiohub.org/public/igem/BBa_J31005_sequence/1

CmR gene
type: SequenceAnnotation
persistent identity: annotation1884999
display id: annotation1884999
version: 1
top level: CmR
location: https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J31005/annotation1884999/range1884999/1
type: Range
persistent identity: range1884999
display id: range1884999
version: 1
top level: CmR
direction: direction/forward
start: 1
end: 660
orientation: inline

https://synbiohub.org/public/igem/BBa_J85139_sequence/1
type: Sequence
persistent identity: BBa J85139 sequence
display id: BBa_J85139_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaacctactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatatactagagaaagaggagaaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P0151_sequence/1
type: Sequence
persistent identity: BBa P0151 sequence
display id: BBa_P0151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacctactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85101_sequence/1
type: Sequence
persistent identity: BBa J85101 sequence
display id: BBa_J85101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K415032_sequence/1
type: Sequence
persistent identity: BBa K415032 sequence
display id: BBa_K415032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31005_sequence/1
type: Sequence
persistent identity: BBa J31005 sequence
display id: BBa_J31005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z