LOCUS BBa_J85205 3569 bp DNA linear 15-Nov-2024 DEFINITION J85203.I13522 ACCESSION BBa_J85205 VERSION BBa_J85205.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 misc_feature 1057..1060 /label=conserved RBS 1053..1064 /label=BBa_B0034 CDS 1071..1745 /label=mrfp1 misc_feature 1752..1776 /label=Help:Barcodes CDS 1071..1751 /label=BBa_E1010 misc_feature 1053..1776 /label=BBa_I13502 misc_feature 1..1776 /label=BBa_J10000 misc_feature 1789..1792 /label=conserved RBS 1785..1796 /label=BBa_B0034 CDS 1806..2422 /label=tetR misc_feature 2423..2456 /label=SsrA misc_feature 2463..2487 /label=Help:Barcodes CDS 1803..2462 /label=BBa_C0040 stem_loop 2507..2550 /label=stem_loop misc_feature 2496..2575 /label=BBa_B0010 terminator 2496..2575 /label=BBa_B0010 stem_loop 2591..2610 /label=T7 TE misc_feature 2617..2617 /label=stop polyA_site 2611..2624 /label=polya misc_feature 2584..2624 /label=BBa_B0012 terminator 2584..2624 /label=BBa_B0012 misc_feature 1785..2624 /label=BBa_P0440 promoter 1..2624 /label=BBa_J85203 misc_feature 2633..2651 /label=TetR 1 misc_feature 2633..2686 /label=BBa_R0040 promoter 2652..2657 /label=-35 misc_feature 2658..2676 /label=TetR 2 promoter 2675..2680 /label=-10 promoter 2633..2686 /label=BBa_R0040 misc_feature 2699..2702 /label=conserved RBS 2695..2706 /label=BBa_B0034 CDS 2713..3432 /label=GFP protein CDS 2713..3432 /label=BBa_E0040 stem_loop 3452..3495 /label=stem_loop misc_feature 3441..3520 /label=BBa_B0010 terminator 3441..3520 /label=BBa_B0010 stem_loop 3536..3555 /label=T7 TE misc_feature 3562..3562 /label=stop polyA_site 3556..3569 /label=polya misc_feature 3529..3569 /label=BBa_B0012 terminator 3529..3569 /label=BBa_B0012 misc_feature 2633..3569 /label=BBa_I13522 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agaaagagga gaaatactag atggcttcct 1081 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg 1141 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg 1201 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc 1261 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga 1321 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg 1381 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac 1441 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg 1501 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc 1561 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta 1621 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc 1681 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg 1741 gtgcttaata acgctgatag tgctagtgta gatcgctact agagaaagag gagaaatact 1801 agatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg 1861 tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta 1921 cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt 1981 tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac 2041 gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac 2101 atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt 2161 tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt 2221 ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa 2281 cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc 2341 aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac 2401 aacttaaatg tgaaagtggg tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat 2461 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc 2521 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2581 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtccctatc 2641 agtgatagag attgacatcc ctatcagtga tagagatact gagcactact agagaaagag 2701 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt 2761 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat 2821 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca 2881 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat 2941 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga 3001 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt 3061 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt 3121 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa 3181 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt 3241 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca 3301 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac 3361 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta 3421 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 3481 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 3541 accttcgggt gggcctttct gcgtttata //