LOCUS       BBa_J85207              2017 bp    DNA     linear       15-Nov-2024
DEFINITION  J85202.J85206
ACCESSION   BBa_J85207
VERSION     BBa_J85207.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     misc_feature    813..816
                     /label=conserved
     RBS             809..820
                     /label=BBa_B0034
     CDS             827..2017
                     /label=tetA(C) forward
     primer_bind     827..850
                     /label=Fwd primer J31007
     primer_bind     1998..2017
                     /label=Rev primer J31007
     misc_feature    2015..2017
                     /label=stop
     CDS             827..2017
                     /label=BBa_J31007
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagaa agaggagaaa tactagatga aatctaacaa
      841 tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
      901 gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta
      961 tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc
     1021 actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat
     1081 cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt
     1141 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     1201 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     1261 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     1321 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     1381 taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     1441 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     1501 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     1561 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     1621 cgtcactggc cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     1681 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     1741 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     1801 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     1861 cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     1921 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     1981 gcgtcgcggt gcatggagcc gggccacctc gacctaa
//