##gff-version 3
##sequence-region BBa_J85209 1 4118
BBa_J85209	.	engineered_region	1	3432	.	+	0	ID=BBa_J85204;Name=BBa_J85204
BBa_J85209	.	promoter	1	2624	.	+	0	ID=BBa_J85203;Name=BBa_J85203;Parent=BBa_J85204
BBa_J85209	.	engineered_region	1	1776	.	+	0	ID=BBa_J10000;Name=BBa_J10000;Parent=BBa_J85203
BBa_J85209	.	engineered_region	1	1044	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_J10000
BBa_J85209	.	engineered_region	46	981	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_J85209	.	terminator	941	981	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_J85209	.	engineered_region	941	981	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85209	.	polyA_site	968	981	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85209	.	stop_codon	974	974	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85209	.	stem_loop	948	967	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85209	.	CDS	64	819	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_J85209	.	point_mutation	555	555	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J85209	.	engineered_tag	820	844	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J85209	.	engineered_region	64	819	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J85209	.	CDS	64	813	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J85209	.	point_mutation	237	237	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J85209	.	engineered_region	64	65	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J85209	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_J85209	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85209	.	terminator	853	932	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_J85209	.	engineered_region	853	932	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85209	.	stem_loop	864	907	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85209	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_J85209	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J85209	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J85209	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J85209	.	promoter	990	1044	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_J85209	.	non_covalent_binding_site	990	1009	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J85209	.	promoter	1031	1036	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J85209	.	promoter	1009	1014	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J85209	.	engineered_region	990	1044	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J85209	.	start_codon	1042	1042	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J85209	.	engineered_region	1053	1776	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_J10000
BBa_J85209	.	ribosome_entry_site	1053	1064	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_J85209	.	conserved_region	1057	1060	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85209	.	CDS	1071	1751	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_J85209	.	CDS	1071	1745	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J85209	.	engineered_tag	1752	1776	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J85209	.	engineered_region	1785	2624	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_J85203
BBa_J85209	.	ribosome_entry_site	1785	1796	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_J85209	.	conserved_region	1789	1792	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85209	.	terminator	2584	2624	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_J85209	.	engineered_region	2584	2624	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85209	.	polyA_site	2611	2624	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85209	.	stop_codon	2617	2617	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85209	.	stem_loop	2591	2610	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85209	.	terminator	2496	2575	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_J85209	.	engineered_region	2496	2575	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85209	.	stem_loop	2507	2550	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85209	.	CDS	1803	2462	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_J85209	.	CDS	1806	2422	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J85209	.	tag	2423	2456	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J85209	.	engineered_tag	2463	2487	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J85209	.	engineered_region	2633	3432	.	+	0	ID=BBa_J85202;Name=BBa_J85202;Parent=BBa_J85204
BBa_J85209	.	ribosome_entry_site	2695	2706	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J85202
BBa_J85209	.	conserved_region	2699	2702	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85209	.	CDS	2713	3432	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J85202
BBa_J85209	.	CDS	2713	3432	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J85209	.	promoter	2633	2686	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J85202
BBa_J85209	.	non_covalent_binding_site	2633	2651	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J85209	.	engineered_region	2633	2686	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J85209	.	non_covalent_binding_site	2658	2676	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J85209	.	promoter	2652	2657	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J85209	.	promoter	2675	2680	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J85209	.	engineered_region	3441	4118	.	+	0	ID=BBa_J85101;Name=BBa_J85101
BBa_J85209	.	ribosome_entry_site	3441	3452	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J85101
BBa_J85209	.	conserved_region	3445	3448	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85209	.	CDS	3459	4118	.	+	0	ID=BBa_J31005;Name=BBa_J31005;Parent=BBa_J85101
BBa_J85209	.	sequence_feature	3459	4118	.	+	0	ID=annotation1884999;Name=CmR gene;Parent=BBa_J31005
>BBa_J85209
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatg
ccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatat
gactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatc
ctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattatt
ctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaa
agaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagt
tttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttcctt
ctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatg
tttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttc
catttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaa
ttgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtt
tgttatagtcgaataaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatg
cgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacg
aaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagtt
ccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttc
aaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagt
tcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttggga
agcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggt
ggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgaca
tcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccac
cggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgtccagattagat
aaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccc
agaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattga
gatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaa
agttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatg
aaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgt
ggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactact
gatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcc
ttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgcttt
agtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
tactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatt
agatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctt
aaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgct
ttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaac
tatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatc
gagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatg
tatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgt
tcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcc
acacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
ttacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatggagaaaaaaatcact
ggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacct
ataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctt
tattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgg
gatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacg
atttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtt
tattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggac
aacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcagg
ttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcaggg
cggggcgtaa