LOCUS       BBa_J85220              1486 bp    DNA     linear       22-Dec-2024
DEFINITION  J85202.J85101 (Ptet GFP CmR)
ACCESSION   BBa_J85220
VERSION     BBa_J85220.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     misc_feature    813..816
                     /label=conserved
     RBS             809..820
                     /label=BBa_B0034
     misc_feature    827..1486
                     /label=CmR gene
     CDS             827..1486
                     /label=BBa_J31005
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagaa agaggagaaa tactagatgg agaaaaaaat
      841 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      901 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      961 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     1021 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
     1081 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     1141 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     1201 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     1261 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     1321 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     1381 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     1441 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaa
//