LOCUS       BBa_J85221              1775 bp    DNA     linear       01-Jun-2024
DEFINITION  J85202.P1003
ACCESSION   BBa_J85221
VERSION     BBa_J85221.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     CDS             957..1775
                     /label=kanamycin resistance
     misc_feature    809..1775
                     /label=BBa_P1003
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagct gatccttcaa ctcagcaaaa gttcgattta
      841 ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
      901 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
      961 gccatattca acgggaaacg tcttgctccc gtccgcgctt aaactccaac atggacgctg
     1021 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc
     1081 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     1141 ccaatgatgt tacagatgag atggtccgtc tcaactggct gacggagttt atgcctctcc
     1201 cgaccatcaa gcattttatc cgtactcctg atgatgcgtg gttactcacc accgcgattc
     1261 ctgggaaaac agccttccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     1321 atgcgctggc cgtgttcctg cgccggttac attcgattcc tgtttgtaat tgtcctttta
     1381 acagcgatcg tgtatttcgt cttgctcagg cgcaatcacg catgaataac ggtttggttg
     1441 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     1501 tgcacaagct cttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     1561 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgggtcggaa
     1621 tcgcagaccg ttaccaggac cttgccattc tttggaactg cctcggtgag ttttctcctt
     1681 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     1741 agtttcattt gatgctcgat gagtttttct aataa
//