LOCUS BBa_J85221 1775 bp DNA linear 31-Oct-2024 DEFINITION J85202.P1003 ACCESSION BBa_J85221 VERSION BBa_J85221.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..800 /label=GFP protein CDS 81..800 /label=BBa_E0040 CDS 957..1775 /label=kanamycin resistance misc_feature 809..1775 /label=BBa_P1003 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 781 atgaactata caaataataa tactagagct gatccttcaa ctcagcaaaa gttcgattta 841 ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata 901 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga 961 gccatattca acgggaaacg tcttgctccc gtccgcgctt aaactccaac atggacgctg 1021 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc 1081 gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg 1141 ccaatgatgt tacagatgag atggtccgtc tcaactggct gacggagttt atgcctctcc 1201 cgaccatcaa gcattttatc cgtactcctg atgatgcgtg gttactcacc accgcgattc 1261 ctgggaaaac agccttccag gtattagaag aatatcctga ttcaggtgaa aatattgttg 1321 atgcgctggc cgtgttcctg cgccggttac attcgattcc tgtttgtaat tgtcctttta 1381 acagcgatcg tgtatttcgt cttgctcagg cgcaatcacg catgaataac ggtttggttg 1441 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa 1501 tgcacaagct cttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 1561 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgggtcggaa 1621 tcgcagaccg ttaccaggac cttgccattc tttggaactg cctcggtgag ttttctcctt 1681 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 1741 agtttcattt gatgctcgat gagtttttct aataa //