LOCUS       BBa_J85223              2584 bp    DNA     linear       15-Nov-2024
DEFINITION  J10000.J85202
ACCESSION   BBa_J85223
VERSION     BBa_J85223.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..65
                     /label=prefix
     misc_feature    237..237
                     /label=T
     misc_feature    555..555
                     /label=A
     CDS             64..813
                     /label=luxR
     misc_feature    64..819
                     /label=BBa_C0062
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             64..819
                     /label=BBa_C0062
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     misc_feature    46..981
                     /label=BBa_I0462
     misc_feature    990..1009
                     /label=LuxR/HSL
     promoter        1009..1014
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1042..1042
                     /label=start
     misc_feature    990..1044
                     /label=BBa_R0062
     promoter        990..1044
                     /label=BBa_R0062
     misc_feature    1..1044
                     /label=BBa_J09855
     misc_feature    1057..1060
                     /label=conserved
     RBS             1053..1064
                     /label=BBa_B0034
     CDS             1071..1745
                     /label=mrfp1
     misc_feature    1752..1776
                     /label=Help:Barcodes
     CDS             1071..1751
                     /label=BBa_E1010
     misc_feature    1053..1776
                     /label=BBa_I13502
     misc_feature    1..1776
                     /label=BBa_J10000
     misc_feature    1785..1803
                     /label=TetR 1
     misc_feature    1785..1838
                     /label=BBa_R0040
     promoter        1804..1809
                     /label=-35
     misc_feature    1810..1828
                     /label=TetR 2
     promoter        1827..1832
                     /label=-10
     promoter        1785..1838
                     /label=BBa_R0040
     misc_feature    1851..1854
                     /label=conserved
     RBS             1847..1858
                     /label=BBa_B0034
     CDS             1865..2584
                     /label=GFP protein
     CDS             1865..2584
                     /label=BBa_E0040
     misc_feature    1785..2584
                     /label=BBa_J85202
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag agaaagagga gaaatactag atggcttcct
     1081 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
     1141 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
     1201 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
     1261 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
     1321 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
     1381 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
     1441 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
     1501 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
     1561 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
     1621 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
     1681 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
     1741 gtgcttaata acgctgatag tgctagtgta gatcgctact agagtcccta tcagtgatag
     1801 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata
     1861 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1921 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1981 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     2041 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     2101 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     2161 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     2221 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     2281 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     2341 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     2401 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     2461 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     2521 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     2581 ataa
//