LOCUS BBa_J85224 4407 bp DNA linear 01-Jun-2024 DEFINITION P1003.J85204 ACCESSION BBa_J85224 VERSION BBa_J85224.1 FEATURES Location/Qualifiers CDS 149..967 /label=kanamycin resistance misc_feature 1..967 /label=BBa_P1003 misc_feature 976..1012 /label=P(Lac) IQ promoter 978..983 /label=-35 promoter 1001..1006 /label=-10 promoter 976..1012 /label=BBa_I14032 misc_feature 1025..1028 /label=conserved RBS 1021..1032 /label=BBa_B0034 misc_feature 1039..1040 /label=prefix misc_feature 1212..1212 /label=T misc_feature 1530..1530 /label=A CDS 1039..1788 /label=luxR misc_feature 1039..1794 /label=BBa_C0062 misc_feature 1795..1819 /label=Help:Barcodes CDS 1039..1794 /label=BBa_C0062 stem_loop 1839..1882 /label=stem_loop misc_feature 1828..1907 /label=BBa_B0010 terminator 1828..1907 /label=BBa_B0010 stem_loop 1923..1942 /label=T7 TE misc_feature 1949..1949 /label=stop polyA_site 1943..1956 /label=polya misc_feature 1916..1956 /label=BBa_B0012 terminator 1916..1956 /label=BBa_B0012 misc_feature 1021..1956 /label=BBa_I0462 misc_feature 1965..1984 /label=LuxR/HSL promoter 1984..1989 /label=-35 promoter 2006..2011 /label=-10 misc_feature 2017..2017 /label=start misc_feature 1965..2019 /label=BBa_R0062 promoter 1965..2019 /label=BBa_R0062 misc_feature 976..2019 /label=BBa_J09855 misc_feature 2032..2035 /label=conserved RBS 2028..2039 /label=BBa_B0034 CDS 2046..2720 /label=mrfp1 misc_feature 2727..2751 /label=Help:Barcodes CDS 2046..2726 /label=BBa_E1010 misc_feature 2028..2751 /label=BBa_I13502 misc_feature 976..2751 /label=BBa_J10000 misc_feature 2764..2767 /label=conserved RBS 2760..2771 /label=BBa_B0034 CDS 2781..3397 /label=tetR misc_feature 3398..3431 /label=SsrA misc_feature 3438..3462 /label=Help:Barcodes CDS 2778..3437 /label=BBa_C0040 stem_loop 3482..3525 /label=stem_loop misc_feature 3471..3550 /label=BBa_B0010 terminator 3471..3550 /label=BBa_B0010 stem_loop 3566..3585 /label=T7 TE misc_feature 3592..3592 /label=stop polyA_site 3586..3599 /label=polya misc_feature 3559..3599 /label=BBa_B0012 terminator 3559..3599 /label=BBa_B0012 misc_feature 2760..3599 /label=BBa_P0440 promoter 976..3599 /label=BBa_J85203 misc_feature 3608..3626 /label=TetR 1 misc_feature 3608..3661 /label=BBa_R0040 promoter 3627..3632 /label=-35 misc_feature 3633..3651 /label=TetR 2 promoter 3650..3655 /label=-10 promoter 3608..3661 /label=BBa_R0040 misc_feature 3674..3677 /label=conserved RBS 3670..3681 /label=BBa_B0034 CDS 3688..4407 /label=GFP protein CDS 3688..4407 /label=BBa_E0040 misc_feature 3608..4407 /label=BBa_J85202 misc_feature 976..4407 /label=BBa_J85204 ORIGIN 1 ctgatccttc aactcagcaa aagttcgatt tattcaacaa agccacgttg tgtctcaaaa 61 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc 121 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc 181 ccgtccgcgc ttaaactcca acatggacgc tgatttatat gggtataaat gggctcgcga 241 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 301 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtccg 361 tctcaactgg ctgacggagt ttatgcctct cccgaccatc aagcatttta tccgtactcc 421 tgatgatgcg tggttactca ccaccgcgat tcctgggaaa acagccttcc aggtattaga 481 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gccgtgttcc tgcgccggtt 541 acattcgatt cctgtttgta attgtccttt taacagcgat cgtgtatttc gtcttgctca 601 ggcgcaatca cgcatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 661 tggctggcct gttgaacaag tctggaaaga aatgcacaag ctcttgccat tctcaccgga 721 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 781 aataggttgt attgatgttg gacgggtcgg aatcgcagac cgttaccagg accttgccat 841 tctttggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 901 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 961 ctaataatac tagagtggtg caaaaccttt cgcggtatgg catgatagcg cctactagag 1021 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat 1081 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa 1141 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct 1201 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat 1261 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg 1321 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa 1381 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga 1441 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg 1501 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata 1561 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca 1621 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc 1681 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt 1741 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga 1801 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 1861 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 1921 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacctgt aggatcgtac 1981 aggtttacgc aagaaaatgg tttgttatag tcgaataaat actagagaaa gaggagaaat 2041 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg 2101 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg 2161 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg 2221 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca 2281 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact 2341 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca 2401 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa 2461 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag 2521 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa 2581 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac 2641 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag 2701 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagaga 2761 aagaggagaa atactagatg tccagattag ataaaagtaa agtgattaac agcgcattag 2821 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag 2881 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct 2941 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct 3001 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg 3061 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa 3121 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca 3181 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta 3241 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg 3301 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat 3361 gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg 3421 ctttagtagc ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca 3481 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 3541 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat 3601 actagagtcc ctatcagtga tagagattga catccctatc agtgatagag atactgagca 3661 ctactagaga aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt 3721 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga 3781 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga 3841 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt 3901 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt 3961 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa 4021 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa 4081 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac 4141 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt 4201 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc 4261 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc 4321 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat 4381 ggcatggatg aactatacaa ataataa //