##gff-version 3
##sequence-region BBa_J85224 1 4407
BBa_J85224	.	engineered_region	976	4407	.	+	0	ID=BBa_J85204;Name=BBa_J85204
BBa_J85224	.	promoter	976	3599	.	+	0	ID=BBa_J85203;Name=BBa_J85203;Parent=BBa_J85204
BBa_J85224	.	engineered_region	976	2751	.	+	0	ID=BBa_J10000;Name=BBa_J10000;Parent=BBa_J85203
BBa_J85224	.	engineered_region	2028	2751	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_J10000
BBa_J85224	.	ribosome_entry_site	2028	2039	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_J85224	.	conserved_region	2032	2035	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85224	.	CDS	2046	2726	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_J85224	.	CDS	2046	2720	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J85224	.	engineered_tag	2727	2751	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J85224	.	engineered_region	976	2019	.	+	0	ID=BBa_J09855;Name=BBa_J09855;Parent=BBa_J10000
BBa_J85224	.	promoter	1965	2019	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_J85224	.	non_covalent_binding_site	1965	1984	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J85224	.	promoter	2006	2011	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J85224	.	engineered_region	1965	2019	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J85224	.	start_codon	2017	2017	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J85224	.	promoter	1984	1989	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J85224	.	promoter	976	1012	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_J85224	.	promoter	978	983	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_J85224	.	promoter	1001	1006	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_J85224	.	operator	976	1012	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_J85224	.	engineered_region	1021	1956	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_J85224	.	terminator	1828	1907	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_J85224	.	stem_loop	1839	1882	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85224	.	engineered_region	1828	1907	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85224	.	ribosome_entry_site	1021	1032	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_J85224	.	conserved_region	1025	1028	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85224	.	CDS	1039	1794	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_J85224	.	CDS	1039	1788	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J85224	.	engineered_region	1039	1794	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J85224	.	engineered_tag	1795	1819	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J85224	.	point_mutation	1212	1212	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J85224	.	engineered_region	1039	1040	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J85224	.	point_mutation	1530	1530	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J85224	.	terminator	1916	1956	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_J85224	.	engineered_region	1916	1956	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85224	.	stop_codon	1949	1949	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85224	.	stem_loop	1923	1942	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85224	.	polyA_site	1943	1956	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85224	.	engineered_region	2760	3599	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_J85203
BBa_J85224	.	terminator	3471	3550	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_J85224	.	stem_loop	3482	3525	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J85224	.	engineered_region	3471	3550	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J85224	.	ribosome_entry_site	2760	2771	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_J85224	.	conserved_region	2764	2767	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85224	.	terminator	3559	3599	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_J85224	.	engineered_region	3559	3599	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J85224	.	stop_codon	3592	3592	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J85224	.	stem_loop	3566	3585	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J85224	.	polyA_site	3586	3599	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J85224	.	CDS	2778	3437	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_J85224	.	engineered_tag	3438	3462	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J85224	.	tag	3398	3431	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J85224	.	CDS	2781	3397	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J85224	.	engineered_region	3608	4407	.	+	0	ID=BBa_J85202;Name=BBa_J85202;Parent=BBa_J85204
BBa_J85224	.	ribosome_entry_site	3670	3681	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J85202
BBa_J85224	.	conserved_region	3674	3677	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J85224	.	promoter	3608	3661	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J85202
BBa_J85224	.	promoter	3627	3632	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J85224	.	non_covalent_binding_site	3633	3651	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J85224	.	engineered_region	3608	3661	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J85224	.	non_covalent_binding_site	3608	3626	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J85224	.	promoter	3650	3655	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J85224	.	CDS	3688	4407	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J85202
BBa_J85224	.	CDS	3688	4407	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J85224	.	engineered_region	1	967	.	+	0	ID=BBa_P1003;Name=BBa_P1003
BBa_J85224	.	CDS	149	967	.	+	0	ID=annotation1897112;Name=kanamycin resistance;Parent=BBa_P1003
>BBa_J85224
ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgca
caagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccata
ttcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcg
cgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacat
ggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccga
ccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggt
attagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcct
gtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttg
atgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccatt
ctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgt
attgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctc
cttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgct
cgatgagtttttctaataatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagagg
agaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataa
tgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcat
tctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaa
taaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgt
aaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacg
gctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgt
gtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaa
cgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatatta
ggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagta
tttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatc
actactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggat
cgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcttcc
tccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcg
aaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgtt
cgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactac
ctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttaccc
aggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggt
tatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatc
aaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttc
agctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagta
cgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagag
gagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcg
aaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcg
ggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctgg
caagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttag
gtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactaga
gaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgct
aaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaag
gtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtc
cgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatc
cctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcac
tggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggt
gatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtca
ctactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccat
gcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagttt
gaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaat
tggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaat
tagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtc
cttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcc
ttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa