LOCUS       BBa_J85700               330 bp    DNA     linear       01-Jun-2024
DEFINITION  TRE-tight
ACCESSION   BBa_J85700
VERSION     BBa_J85700.1  
FEATURES             Location/Qualifiers
     misc_feature    30..35
                     /label=Bind_Site_1
     misc_feature    46..63
                     /label=tetO reg
     misc_feature    81..96
                     /label=tetO reg
     misc_feature    117..134
                     /label=tetO reg
     misc_feature    153..170
                     /label=tetO reg
     misc_feature    188..205
                     /label=tetO reg
     misc_feature    224..241
                     /label=tetO reg
     promoter        256..313
                     /label=CMV-minimal
     misc_feature    314..315
                     /label=Bind_Site_2
ORIGIN
        1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcaaaggg cgaattcgac
//