##gff-version 3
##sequence-region BBa_J85900 1 2630
BBa_J85900	.	promoter	1	330	.	+	0	ID=BBa_J85700;Name=BBa_J85700
BBa_J85900	.	operator	224	241	.	+	0	ID=annotation2125936;Name=tetO reg;Parent=BBa_J85700
BBa_J85900	.	operator	153	170	.	+	0	ID=annotation2125934;Name=tetO reg;Parent=BBa_J85700
BBa_J85900	.	non_covalent_binding_site	314	315	.	+	0	ID=annotation2125938;Name=Bind_Site_2;Parent=BBa_J85700
BBa_J85900	.	operator	46	63	.	+	0	ID=annotation2125931;Name=tetO reg;Parent=BBa_J85700
BBa_J85900	.	operator	188	205	.	+	0	ID=annotation2125935;Name=tetO reg;Parent=BBa_J85700
BBa_J85900	.	operator	117	134	.	+	0	ID=annotation2125933;Name=tetO reg;Parent=BBa_J85700
BBa_J85900	.	operator	81	96	.	+	0	ID=annotation2125932;Name=tetO reg;Parent=BBa_J85700
BBa_J85900	.	non_covalent_binding_site	30	35	.	+	0	ID=annotation2125930;Name=Bind_Site_1;Parent=BBa_J85700
BBa_J85900	.	promoter	256	313	.	+	0	ID=annotation2125937;Name=CMV-minimal;Parent=BBa_J85700
BBa_J85900	.	CDS	353	2630	.	+	0	ID=BBa_J85800;Name=BBa_J85800
BBa_J85900	.	stem_loop	2261	2609	.	+	0	ID=annotation2125947;Name=miRNA FF4;Parent=BBa_J85800
BBa_J85900	.	CDS	360	1475	.	+	0	ID=annotation2125943;Name=LacI;Parent=BBa_J85800
BBa_J85900	.	CDS	1530	2249	.	+	0	ID=annotation2125945;Name=EBFP2;Parent=BBa_J85800
BBa_J85900	.	sequence_feature	2250	2260	.	+	0	ID=annotation2125946;Name=splice site 1;Parent=BBa_J85800
BBa_J85900	.	tag	1476	1529	.	+	0	ID=annotation2125944;Name=T2A;Parent=BBa_J85800
BBa_J85900	.	sequence_feature	2610	2630	.	+	0	ID=annotation2125948;Name=splice site 2;Parent=BBa_J85800
BBa_J85900	.	tag	331	352	.	+	0	ID=BBa_J85600;Name=BBa_J85600
BBa_J85900	.	sequence_feature	332	352	.	+	0	ID=annotation2125966;Name=attB1;Parent=BBa_J85600
>BBa_J85900
gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttac
tccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctat
cagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgata
gagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaa
agggcgaattcgacccaagtttgtacaaaaaagcagtcccaccatgaaaccagtaacgttatacgatgtcgcagagtat
gccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtgg
aagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattgg
cgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggt
gccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaac
gcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttcc
ggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggc
gtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgc
gtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccat
gtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatg
gcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgata
ccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtgga
ccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaacc
accctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttccc
gactggaaagcgggcagagcagcctgaggcctcctaagaagaagaggaaggttgagggccgcggcagcctgctgacctg
cggcgacgtggaggaaaaccccggccccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtc
gagctggacggcgacgtaaacggccacaagttcagcgtgaggggcgagggcgagggcgatgccaccaacggcaagctga
ccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgagccacggcgtgca
gtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggag
cgcaccatcttcttcaaggacgacggcacctacaagacccgcgccgaggtgaagttcgagggcgacaccctagtgaacc
gcatcgagctgaagggcgtcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacttcaacagcca
caacatctatatcatggccgtcaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacgtggaggacggc
agcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacagccactacc
tgagcacccagtccgtgctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttccgcaccgccgc
cgggatcactctcggcatggacgagctgtacaagtaagaggtgagtatgtgctcgcttcggcagcacatatactatgtt
gaatgaggcttcagtactttacagaatcgttgcctgcacatcttggaaacacttgctgggattacttcttcaggttaac
ccaacagaaggctcgagtgctgttgacagtgagcgccgcttgaagtttttaattaaatagtgaagccacagatgtattt
aattaaagacttcaagcggtgcctactgcctcggagaattcaaggggctactttaggagcaattatcttgtttactaaa
actgaataccttgctatctctttgatacatttttacaaagctgaattaaaatggtataaattaaatcacttttttcaat
tgtttccttttttttcctcaggg