##gff-version 3
##sequence-region BBa_J85950 1 7661
BBa_J85950	.	engineered_region	1	2630	.	+	0	ID=BBa_J85900;Name=BBa_J85900
BBa_J85950	.	CDS	353	2630	.	+	0	ID=BBa_J85800;Name=BBa_J85800;Parent=BBa_J85900
BBa_J85950	.	tag	1476	1529	.	+	0	ID=annotation2125944;Name=T2A;Parent=BBa_J85800
BBa_J85950	.	stem_loop	2261	2609	.	+	0	ID=annotation2125947;Name=miRNA FF4;Parent=BBa_J85800
BBa_J85950	.	sequence_feature	2250	2260	.	+	0	ID=annotation2125946;Name=splice site 1;Parent=BBa_J85800
BBa_J85950	.	CDS	360	1475	.	+	0	ID=annotation2125943;Name=LacI;Parent=BBa_J85800
BBa_J85950	.	CDS	1530	2249	.	+	0	ID=annotation2125945;Name=EBFP2;Parent=BBa_J85800
BBa_J85950	.	sequence_feature	2610	2630	.	+	0	ID=annotation2125948;Name=splice site 2;Parent=BBa_J85800
BBa_J85950	.	promoter	1	330	.	+	0	ID=BBa_J85700;Name=BBa_J85700;Parent=BBa_J85900
BBa_J85950	.	non_covalent_binding_site	30	35	.	+	0	ID=annotation2125930;Name=Bind_Site_1;Parent=BBa_J85700
BBa_J85950	.	operator	224	241	.	+	0	ID=annotation2125936;Name=tetO reg;Parent=BBa_J85700
BBa_J85950	.	operator	81	96	.	+	0	ID=annotation2125932;Name=tetO reg;Parent=BBa_J85700
BBa_J85950	.	non_covalent_binding_site	314	315	.	+	0	ID=annotation2125938;Name=Bind_Site_2;Parent=BBa_J85700
BBa_J85950	.	operator	46	63	.	+	0	ID=annotation2125931;Name=tetO reg;Parent=BBa_J85700
BBa_J85950	.	operator	117	134	.	+	0	ID=annotation2125933;Name=tetO reg;Parent=BBa_J85700
BBa_J85950	.	operator	188	205	.	+	0	ID=annotation2125935;Name=tetO reg;Parent=BBa_J85700
BBa_J85950	.	promoter	256	313	.	+	0	ID=annotation2125937;Name=CMV-minimal;Parent=BBa_J85700
BBa_J85950	.	operator	153	170	.	+	0	ID=annotation2125934;Name=tetO reg;Parent=BBa_J85700
BBa_J85950	.	tag	331	352	.	+	0	ID=BBa_J85600;Name=BBa_J85600;Parent=BBa_J85900
BBa_J85950	.	sequence_feature	332	352	.	+	0	ID=annotation2125966;Name=attB1;Parent=BBa_J85600
BBa_J85950	.	terminator	2631	2675	.	+	0	ID=BBa_J85602;Name=BBa_J85602
BBa_J85950	.	tag	2631	2675	.	+	0	ID=annotation2126021;Name=scar 2;Parent=BBa_J85602
BBa_J85950	.	terminator	4873	4917	.	+	0	ID=BBa_J85603;Name=BBa_J85603
BBa_J85950	.	tag	4873	4917	.	+	0	ID=annotation2126022;Name=scar 3;Parent=BBa_J85603
BBa_J85950	.	engineered_region	4918	7661	.	+	0	ID=BBa_J85901;Name=BBa_J85901
BBa_J85950	.	CDS	6158	7661	.	+	0	ID=BBa_J85801;Name=BBa_J85801;Parent=BBa_J85901
BBa_J85950	.	CDS	6167	6871	.	+	0	ID=annotation2125987;Name=rtTA3;Parent=BBa_J85801
BBa_J85950	.	CDS	6962	7657	.	+	0	ID=annotation2125990;Name=mKate2;Parent=BBa_J85801
BBa_J85950	.	tag	6938	6961	.	+	0	ID=annotation2125989;Name=NLS;Parent=BBa_J85801
BBa_J85950	.	tag	6878	6931	.	+	0	ID=annotation2125988;Name=2A;Parent=BBa_J85801
BBa_J85950	.	promoter	4918	6135	.	+	0	ID=BBa_J85701;Name=BBa_J85701;Parent=BBa_J85901
BBa_J85950	.	promoter	4940	6113	.	+	0	ID=annotation2125986;Name=pHef1A;Parent=BBa_J85701
BBa_J85950	.	tag	6136	6157	.	+	0	ID=BBa_J85600;Name=BBa_J85600;Parent=BBa_J85901
BBa_J85950	.	sequence_feature	6137	6157	.	+	0	ID=annotation2125966;Name=attB1;Parent=BBa_J85600
BBa_J85950	.	engineered_region	2676	4872	.	+	0	ID=BBa_J85902;Name=BBa_J85902
BBa_J85950	.	promoter	2676	3990	.	+	0	ID=BBa_J85702;Name=BBa_J85702;Parent=BBa_J85902
BBa_J85950	.	operator	2962	2981	.	+	0	ID=annotation2126002;Name=lacOid;Parent=BBa_J85702
BBa_J85950	.	promoter	2691	3965	.	+	0	ID=annotation2126000;Name=Hef1a;Parent=BBa_J85702
BBa_J85950	.	operator	2891	2911	.	+	0	ID=annotation2126001;Name=lacO1;Parent=BBa_J85702
BBa_J85950	.	tag	3991	4012	.	+	0	ID=BBa_J85600;Name=BBa_J85600;Parent=BBa_J85902
BBa_J85950	.	sequence_feature	3992	4012	.	+	0	ID=annotation2125966;Name=attB1;Parent=BBa_J85600
BBa_J85950	.	CDS	4013	4872	.	+	0	ID=BBa_J85802;Name=BBa_J85802;Parent=BBa_J85902
BBa_J85950	.	stem_loop	4779	4800	.	+	0	ID=annotation2126004;Name=FF4;Parent=BBa_J85802
BBa_J85950	.	stem_loop	4801	4822	.	+	0	ID=annotation2126005;Name=FF4;Parent=BBa_J85802
BBa_J85950	.	CDS	4048	4767	.	+	0	ID=annotation2126003;Name=EYFP;Parent=BBa_J85802
BBa_J85950	.	stem_loop	4845	4866	.	+	0	ID=annotation2126007;Name=FF4;Parent=BBa_J85802
BBa_J85950	.	stem_loop	4823	4844	.	+	0	ID=annotation2126006;Name=FF4;Parent=BBa_J85802
>BBa_J85950
gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttac
tccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctat
cagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgata
gagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaa
agggcgaattcgacccaagtttgtacaaaaaagcagtcccaccatgaaaccagtaacgttatacgatgtcgcagagtat
gccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtgg
aagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattgg
cgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggt
gccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaac
gcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttcc
ggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggc
gtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgc
gtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccat
gtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatg
gcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgata
ccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtgga
ccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaacc
accctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttccc
gactggaaagcgggcagagcagcctgaggcctcctaagaagaagaggaaggttgagggccgcggcagcctgctgacctg
cggcgacgtggaggaaaaccccggccccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtc
gagctggacggcgacgtaaacggccacaagttcagcgtgaggggcgagggcgagggcgatgccaccaacggcaagctga
ccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgagccacggcgtgca
gtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggag
cgcaccatcttcttcaaggacgacggcacctacaagacccgcgccgaggtgaagttcgagggcgacaccctagtgaacc
gcatcgagctgaagggcgtcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacttcaacagcca
caacatctatatcatggccgtcaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacgtggaggacggc
agcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacagccactacc
tgagcacccagtccgtgctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttccgcaccgccgc
cgggatcactctcggcatggacgagctgtacaagtaagaggtgagtatgtgctcgcttcggcagcacatatactatgtt
gaatgaggcttcagtactttacagaatcgttgcctgcacatcttggaaacacttgctgggattacttcttcaggttaac
ccaacagaaggctcgagtgctgttgacagtgagcgccgcttgaagtttttaattaaatagtgaagccacagatgtattt
aattaaagacttcaagcggtgcctactgcctcggagaattcaaggggctactttaggagcaattatcttgtttactaaa
actgaataccttgctatctctttgatacatttttacaaagctgaattaaaatggtataaattaaatcacttttttcaat
tgtttccttttttttcctcagggtttaccgcatctagacctaactgagatactgccatagacgactacgctccgaattc
taattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaa
ttgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgag
ggtgggggagaaccgtatataagtgcagtagtcgccgtgaagctagaattgtgagcggataacaattccacagtcgacc
ctaggttgtgtcgcgagtgttggatcccagctgacaccaattgtgagcgctcacaattgctagccgttctttttcgcaa
cgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgc
gtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagtt
cgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcg
aatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcg
acgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgg
gcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacg
ggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaagg
ctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggagga
cgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatg
tgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttgg
ggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgt
aattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttt
tcttccatttcaggtgaagacttgatccccggaattcgacccaagtttgtacaaaaaagcagttcttccatttcaggtg
gatccaccggtcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacg
gcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagtt
catctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcc
cgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatct
tcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagct
gaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctat
atcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagc
tcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctacca
gtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcact
ctcggcatggacgagctgtacaagtaaagcggcgcgccccgcttgaagtctttaattaaaccgcttgaagtctttaatt
aaaccgcttgaagtctttaattaaaccgcttgaagtctttaattaaagctagtccatccctctgggccttagatagccg
gatacagtgactttgaaaggctccgaattcgcccttattaattggctccggtgcccgtcagtgggcagagcgcacatcg
cccacagtccccgagaagttgtggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactggg
aaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaa
cgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacg
ggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaa
gtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctg
gggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttt
tgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcgg
tttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggcc
accgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccg
ccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcaggga
gctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctc
agccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacg
tcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccag
cttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagt
ggttcaaagtttttttcttccatttcaggtggatcccaagggcgaattcgacccaagtttgtacaaaaaagcaggacgc
caccatgtctaggctggacaagagcaaagtcataaacggagctctggaattactcaatggtgtcggtatcgaaggcctg
acgacaaggaaactcgctcaaaagctgggagttgagcagcctaccctgtactggcacgtgaagaacaagcgggccctgc
tcgatgccctgccaatcgagatgctggacaggcatcatacccacttctgccccctggaaggcgagtcatggcaagactt
tctgcggaacaacgccaagtcataccgctgtgctctcctctcacatcgcgacggggctaaagtgcatctcggcacccgc
ccaacagagaaacagtacgaaaccctggaaaatcagctcgcgttcctgtgtcagcaaggcttctccctggagaacgcac
tgtacgctctgtccgccgtgggccactttacactgggctgcgtattggaggaacaggagcatcaagtagcaaaagagga
aagagagacacctaccaccgattctatgcccccacttctgagacaagcaattgagctgttcgaccggcagggagccgaa
cctgccttccttttcggcctggaactaatcatatgtggcctggagaaacagctaaagtgcgaaagcggcgggccgaccg
acgcccttgacgattttgacttagacatgctcccagccgatgcccttgacgattttgaccttgacatgctccccgggga
attggagggccgcggcagcctgctgacctgcggcgacgtggaggaaaaccccggccccatggtgccaccaaaaaaaaaa
cgcaaagttgtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccact
tcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccc
tctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatc
cccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctga
ccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaa
cggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaa
ggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaaga
aacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaaga
gacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatacc