BBa_J85950
type: ComponentDefinition
persistent identity: BBa J85950
display id: BBa_J85950
version: 1
was generated by: iGEM to SBOL conversion
description: LacI inverter regulated by TRE
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: various
owned by: james
owned by: myers
mutable description: TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4-scar2-rtTa3-2A-NLS-mKate2-scar3-EYFP-FF4x4 Composite part that forms an mammalian LacI inverter regulated by TRE
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Inverter
role: SO:0000804
component: BBa_J85900
component: BBa_J85901
component: BBa_J85603
component: BBa_J85902
component: BBa_J85602
sequence annotation: BBa_J85900
sequence annotation: BBa_J85602
sequence annotation: BBa_J85902
sequence annotation: BBa_J85603
sequence annotation: BBa_J85901
sequence: https://synbiohub.org/public/igem/BBa_J85950_sequence/1

BBa_J85900
type: Component
persistent identity: component2126089
display id: component2126089
version: 1
top level: BBa_J85950
definition: BBa_J85900
access: public

BBa_J85901
type: Component
persistent identity: component2126116
display id: component2126116
version: 1
top level: BBa_J85950
definition: BBa_J85901
access: public

BBa_J85603
type: Component
persistent identity: component2126106
display id: component2126106
version: 1
top level: BBa_J85950
definition: BBa_J85603
access: public

BBa_J85902
type: Component
persistent identity: component2126104
display id: component2126104
version: 1
top level: BBa_J85950
definition: BBa_J85902
access: public

BBa_J85602
type: Component
persistent identity: component2126091
display id: component2126091
version: 1
top level: BBa_J85950
definition: BBa_J85602
access: public

BBa_J85900
type: SequenceAnnotation
persistent identity: annotation2126089
display id: annotation2126089
version: 1
top level: BBa_J85950
location: https://synbiohub.org/public/igem/BBa_J85950/annotation2126089/range2126089/1
component: BBa_J85900

https://synbiohub.org/public/igem/BBa_J85950/annotation2126089/range2126089/1
type: Range
persistent identity: range2126089
display id: range2126089
version: 1
top level: BBa_J85950
direction: direction/reverse
start: 1
end: 2630
orientation: inline

BBa_J85602
type: SequenceAnnotation
persistent identity: annotation2126091
display id: annotation2126091
version: 1
top level: BBa_J85950
location: https://synbiohub.org/public/igem/BBa_J85950/annotation2126091/range2126091/1
component: BBa_J85602

https://synbiohub.org/public/igem/BBa_J85950/annotation2126091/range2126091/1
type: Range
persistent identity: range2126091
display id: range2126091
version: 1
top level: BBa_J85950
direction: direction/reverse
start: 2631
end: 2675
orientation: inline

BBa_J85902
type: SequenceAnnotation
persistent identity: annotation2126104
display id: annotation2126104
version: 1
top level: BBa_J85950
location: https://synbiohub.org/public/igem/BBa_J85950/annotation2126104/range2126104/1
component: BBa_J85902

https://synbiohub.org/public/igem/BBa_J85950/annotation2126104/range2126104/1
type: Range
persistent identity: range2126104
display id: range2126104
version: 1
top level: BBa_J85950
direction: direction/reverse
start: 2676
end: 4872
orientation: inline

BBa_J85603
type: SequenceAnnotation
persistent identity: annotation2126106
display id: annotation2126106
version: 1
top level: BBa_J85950
location: https://synbiohub.org/public/igem/BBa_J85950/annotation2126106/range2126106/1
component: BBa_J85603

https://synbiohub.org/public/igem/BBa_J85950/annotation2126106/range2126106/1
type: Range
persistent identity: range2126106
display id: range2126106
version: 1
top level: BBa_J85950
direction: direction/reverse
start: 4873
end: 4917
orientation: inline

BBa_J85901
type: SequenceAnnotation
persistent identity: annotation2126116
display id: annotation2126116
version: 1
top level: BBa_J85950
location: https://synbiohub.org/public/igem/BBa_J85950/annotation2126116/range2126116/1
component: BBa_J85901

https://synbiohub.org/public/igem/BBa_J85950/annotation2126116/range2126116/1
type: Range
persistent identity: range2126116
display id: range2126116
version: 1
top level: BBa_J85950
direction: direction/reverse
start: 4918
end: 7661
orientation: inline

BBa_J85900
type: ComponentDefinition
persistent identity: BBa J85900
display id: BBa_J85900
version: 1
was generated by: iGEM to SBOL conversion
description: TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4
owned by: james
owned by: myers
mutable description: TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J85700
component: BBa_J85800
component: BBa_J85600
sequence annotation: BBa_J85700
sequence annotation: BBa_J85600
sequence annotation: BBa_J85800
sequence: https://synbiohub.org/public/igem/BBa_J85900_sequence/1

BBa_J85700
type: Component
persistent identity: component2125976
display id: component2125976
version: 1
top level: BBa_J85900
definition: BBa_J85700
access: public

BBa_J85800
type: Component
persistent identity: component2125985
display id: component2125985
version: 1
top level: BBa_J85900
definition: BBa_J85800
access: public

BBa_J85600
type: Component
persistent identity: component2125978
display id: component2125978
version: 1
top level: BBa_J85900
definition: BBa_J85600
access: public

BBa_J85700
type: SequenceAnnotation
persistent identity: annotation2125976
display id: annotation2125976
version: 1
top level: BBa_J85900
location: https://synbiohub.org/public/igem/BBa_J85900/annotation2125976/range2125976/1
component: BBa_J85700

https://synbiohub.org/public/igem/BBa_J85900/annotation2125976/range2125976/1
type: Range
persistent identity: range2125976
display id: range2125976
version: 1
top level: BBa_J85900
direction: direction/reverse
start: 1
end: 330
orientation: inline

BBa_J85600
type: SequenceAnnotation
persistent identity: annotation2125978
display id: annotation2125978
version: 1
top level: BBa_J85900
location: https://synbiohub.org/public/igem/BBa_J85900/annotation2125978/range2125978/1
component: BBa_J85600

https://synbiohub.org/public/igem/BBa_J85900/annotation2125978/range2125978/1
type: Range
persistent identity: range2125978
display id: range2125978
version: 1
top level: BBa_J85900
direction: direction/reverse
start: 331
end: 352
orientation: inline

BBa_J85800
type: SequenceAnnotation
persistent identity: annotation2125985
display id: annotation2125985
version: 1
top level: BBa_J85900
location: https://synbiohub.org/public/igem/BBa_J85900/annotation2125985/range2125985/1
component: BBa_J85800

https://synbiohub.org/public/igem/BBa_J85900/annotation2125985/range2125985/1
type: Range
persistent identity: range2125985
display id: range2125985
version: 1
top level: BBa_J85900
direction: direction/reverse
start: 353
end: 2630
orientation: inline

BBa_J85901
type: ComponentDefinition
persistent identity: BBa J85901
display id: BBa_J85901
version: 1
was generated by: iGEM to SBOL conversion
description: Hef1a-B1-rtTA3-2A-NLS-mKate2
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: various
owned by: james
owned by: myers
mutable description: Hef1a-B1-rtTA3-2A-NLS-mKate2 Constitutive promoter Hef1a regulates production of rtTA3 transcriptional activator and nuclear-localized FP mKate
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J85801
component: BBa_J85600
component: BBa_J85701
sequence annotation: BBa_J85701
sequence annotation: BBa_J85600
sequence annotation: BBa_J85801
sequence: https://synbiohub.org/public/igem/BBa_J85901_sequence/1

BBa_J85801
type: Component
persistent identity: component2125999
display id: component2125999
version: 1
top level: BBa_J85901
definition: BBa_J85801
access: public

BBa_J85600
type: Component
persistent identity: component2125994
display id: component2125994
version: 1
top level: BBa_J85901
definition: BBa_J85600
access: public

BBa_J85701
type: Component
persistent identity: component2125992
display id: component2125992
version: 1
top level: BBa_J85901
definition: BBa_J85701
access: public

BBa_J85701
type: SequenceAnnotation
persistent identity: annotation2125992
display id: annotation2125992
version: 1
top level: BBa_J85901
location: https://synbiohub.org/public/igem/BBa_J85901/annotation2125992/range2125992/1
component: BBa_J85701

https://synbiohub.org/public/igem/BBa_J85901/annotation2125992/range2125992/1
type: Range
persistent identity: range2125992
display id: range2125992
version: 1
top level: BBa_J85901
direction: direction/reverse
start: 1
end: 1218
orientation: inline

BBa_J85600
type: SequenceAnnotation
persistent identity: annotation2125994
display id: annotation2125994
version: 1
top level: BBa_J85901
location: https://synbiohub.org/public/igem/BBa_J85901/annotation2125994/range2125994/1
component: BBa_J85600

https://synbiohub.org/public/igem/BBa_J85901/annotation2125994/range2125994/1
type: Range
persistent identity: range2125994
display id: range2125994
version: 1
top level: BBa_J85901
direction: direction/reverse
start: 1219
end: 1240
orientation: inline

BBa_J85801
type: SequenceAnnotation
persistent identity: annotation2125999
display id: annotation2125999
version: 1
top level: BBa_J85901
location: https://synbiohub.org/public/igem/BBa_J85901/annotation2125999/range2125999/1
component: BBa_J85801

https://synbiohub.org/public/igem/BBa_J85901/annotation2125999/range2125999/1
type: Range
persistent identity: range2125999
display id: range2125999
version: 1
top level: BBa_J85901
direction: direction/reverse
start: 1241
end: 2744
orientation: inline

BBa_J85902
type: ComponentDefinition
persistent identity: BBa J85902
display id: BBa_J85902
version: 1
was generated by: iGEM to SBOL conversion
description: Hef1a-LacO1Oid-B1-EYFP-FF4x4
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: various
owned by: james
owned by: myers
mutable description: Hef1a-LacO1Oid-B1-EYFP-FF4x4 Features: * Human elongation factor 1 alpha constitutive promoter * Lac operator 1 * Lac operator ideal * (attB1 scar from recombination assembly) * Enhanced yellow FP * microRNA target site FF4 (four copies)
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: see basic parts
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J85702
component: BBa_J85802
component: BBa_J85600
sequence annotation: BBa_J85702
sequence annotation: BBa_J85600
sequence annotation: BBa_J85802
sequence: https://synbiohub.org/public/igem/BBa_J85902_sequence/1

BBa_J85702
type: Component
persistent identity: component2126011
display id: component2126011
version: 1
top level: BBa_J85902
definition: BBa_J85702
access: public

BBa_J85802
type: Component
persistent identity: component2126019
display id: component2126019
version: 1
top level: BBa_J85902
definition: BBa_J85802
access: public

BBa_J85600
type: Component
persistent identity: component2126013
display id: component2126013
version: 1
top level: BBa_J85902
definition: BBa_J85600
access: public

BBa_J85702
type: SequenceAnnotation
persistent identity: annotation2126011
display id: annotation2126011
version: 1
top level: BBa_J85902
location: https://synbiohub.org/public/igem/BBa_J85902/annotation2126011/range2126011/1
component: BBa_J85702

https://synbiohub.org/public/igem/BBa_J85902/annotation2126011/range2126011/1
type: Range
persistent identity: range2126011
display id: range2126011
version: 1
top level: BBa_J85902
direction: direction/reverse
start: 1
end: 1315
orientation: inline

BBa_J85600
type: SequenceAnnotation
persistent identity: annotation2126013
display id: annotation2126013
version: 1
top level: BBa_J85902
location: https://synbiohub.org/public/igem/BBa_J85902/annotation2126013/range2126013/1
component: BBa_J85600

https://synbiohub.org/public/igem/BBa_J85902/annotation2126013/range2126013/1
type: Range
persistent identity: range2126013
display id: range2126013
version: 1
top level: BBa_J85902
direction: direction/reverse
start: 1316
end: 1337
orientation: inline

BBa_J85802
type: SequenceAnnotation
persistent identity: annotation2126019
display id: annotation2126019
version: 1
top level: BBa_J85902
location: https://synbiohub.org/public/igem/BBa_J85902/annotation2126019/range2126019/1
component: BBa_J85802

https://synbiohub.org/public/igem/BBa_J85902/annotation2126019/range2126019/1
type: Range
persistent identity: range2126019
display id: range2126019
version: 1
top level: BBa_J85902
direction: direction/reverse
start: 1338
end: 2197
orientation: inline

BBa_J85602
type: ComponentDefinition
persistent identity: BBa J85602
display id: BBa_J85602
version: 1
was generated by: iGEM to SBOL conversion
description: scar 2
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: synthesized
owned by: james
owned by: myers
mutable description: Multipart assembly scar2
discontinued: false
dominant: false
experience: experience/None
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: minimal homology
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: scar 2
sequence: https://synbiohub.org/public/igem/BBa_J85602_sequence/1

scar 2
type: SequenceAnnotation
persistent identity: annotation2126021
display id: annotation2126021
version: 1
top level: BBa_J85602
location: https://synbiohub.org/public/igem/BBa_J85602/annotation2126021/range2126021/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J85602/annotation2126021/range2126021/1
type: Range
persistent identity: range2126021
display id: range2126021
version: 1
top level: BBa_J85602
direction: direction/forward
start: 1
end: 45
orientation: inline

BBa_J85603
type: ComponentDefinition
persistent identity: BBa J85603
display id: BBa_J85603
version: 1
was generated by: iGEM to SBOL conversion
description: scar 3
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: synthesized
owned by: james
owned by: myers
mutable description: Multipart assembly scar 3
discontinued: false
dominant: false
experience: experience/None
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: minimize homology
star: false
creator: Ron Weiss
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: scar 3
sequence: https://synbiohub.org/public/igem/BBa_J85603_sequence/1

scar 3
type: SequenceAnnotation
persistent identity: annotation2126022
display id: annotation2126022
version: 1
top level: BBa_J85603
location: https://synbiohub.org/public/igem/BBa_J85603/annotation2126022/range2126022/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J85603/annotation2126022/range2126022/1
type: Range
persistent identity: range2126022
display id: range2126022
version: 1
top level: BBa_J85603
direction: direction/forward
start: 1
end: 45
orientation: inline

BBa_J85700
type: ComponentDefinition
persistent identity: BBa J85700
display id: BBa_J85700
version: 1
was generated by: iGEM to SBOL conversion
description: TRE-tight
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: Revised version of TetON system
owned by: james
owned by: myers
mutable description: Tet response element; tightly regulated.
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Ron Weiss
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Bind_Site_1
sequence annotation: tetO reg
sequence annotation: CMV-minimal
sequence annotation: Bind_Site_2
sequence: https://synbiohub.org/public/igem/BBa_J85700_sequence/1

Bind_Site_1
type: SequenceAnnotation
persistent identity: annotation2125930
display id: annotation2125930
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125930/range2125930/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J85700/annotation2125930/range2125930/1
type: Range
persistent identity: range2125930
display id: range2125930
version: 1
top level: BBa_J85700
direction: direction/forward
start: 30
end: 35
orientation: inline

tetO reg
type: SequenceAnnotation
persistent identity: annotation2125931
display id: annotation2125931
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125931/range2125931/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J85700/annotation2125931/range2125931/1
type: Range
persistent identity: range2125931
display id: range2125931
version: 1
top level: BBa_J85700
direction: direction/forward
start: 46
end: 63
orientation: inline

tetO reg
type: SequenceAnnotation
persistent identity: annotation2125932
display id: annotation2125932
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125932/range2125932/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J85700/annotation2125932/range2125932/1
type: Range
persistent identity: range2125932
display id: range2125932
version: 1
top level: BBa_J85700
direction: direction/forward
start: 81
end: 96
orientation: inline

tetO reg
type: SequenceAnnotation
persistent identity: annotation2125933
display id: annotation2125933
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125933/range2125933/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J85700/annotation2125933/range2125933/1
type: Range
persistent identity: range2125933
display id: range2125933
version: 1
top level: BBa_J85700
direction: direction/forward
start: 117
end: 134
orientation: inline

tetO reg
type: SequenceAnnotation
persistent identity: annotation2125934
display id: annotation2125934
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125934/range2125934/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J85700/annotation2125934/range2125934/1
type: Range
persistent identity: range2125934
display id: range2125934
version: 1
top level: BBa_J85700
direction: direction/forward
start: 153
end: 170
orientation: inline

tetO reg
type: SequenceAnnotation
persistent identity: annotation2125935
display id: annotation2125935
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125935/range2125935/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J85700/annotation2125935/range2125935/1
type: Range
persistent identity: range2125935
display id: range2125935
version: 1
top level: BBa_J85700
direction: direction/forward
start: 188
end: 205
orientation: inline

tetO reg
type: SequenceAnnotation
persistent identity: annotation2125936
display id: annotation2125936
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125936/range2125936/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_J85700/annotation2125936/range2125936/1
type: Range
persistent identity: range2125936
display id: range2125936
version: 1
top level: BBa_J85700
direction: direction/forward
start: 224
end: 241
orientation: inline

CMV-minimal
type: SequenceAnnotation
persistent identity: annotation2125937
display id: annotation2125937
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125937/range2125937/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J85700/annotation2125937/range2125937/1
type: Range
persistent identity: range2125937
display id: range2125937
version: 1
top level: BBa_J85700
direction: direction/forward
start: 256
end: 313
orientation: inline

Bind_Site_2
type: SequenceAnnotation
persistent identity: annotation2125938
display id: annotation2125938
version: 1
top level: BBa_J85700
location: https://synbiohub.org/public/igem/BBa_J85700/annotation2125938/range2125938/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J85700/annotation2125938/range2125938/1
type: Range
persistent identity: range2125938
display id: range2125938
version: 1
top level: BBa_J85700
direction: direction/forward
start: 314
end: 315
orientation: inline

BBa_J85800
type: ComponentDefinition
persistent identity: BBa J85800
display id: BBa_J85800
version: 1
was generated by: iGEM to SBOL conversion
description: LacI-T2A-EBFP2-FF5-miRFF4
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: fusion of several basic parts
owned by: james
owned by: myers
mutable description: LacI-T2A-EBFP2-FF5-miRFF4 Features: * transcriptional repressor LacI * 2A translational fusion tag * enhanced blue fluorescent protein EBFP2 * microRNA target site FF5 * microRNA FF4
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: LacI
sequence annotation: T2A
sequence annotation: EBFP2
sequence annotation: splice site 1
sequence annotation: miRNA FF4
sequence annotation: splice site 2
sequence: https://synbiohub.org/public/igem/BBa_J85800_sequence/1

LacI
type: SequenceAnnotation
persistent identity: annotation2125943
display id: annotation2125943
version: 1
top level: BBa_J85800
location: https://synbiohub.org/public/igem/BBa_J85800/annotation2125943/range2125943/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J85800/annotation2125943/range2125943/1
type: Range
persistent identity: range2125943
display id: range2125943
version: 1
top level: BBa_J85800
direction: direction/forward
start: 8
end: 1123
orientation: inline

T2A
type: SequenceAnnotation
persistent identity: annotation2125944
display id: annotation2125944
version: 1
top level: BBa_J85800
location: https://synbiohub.org/public/igem/BBa_J85800/annotation2125944/range2125944/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J85800/annotation2125944/range2125944/1
type: Range
persistent identity: range2125944
display id: range2125944
version: 1
top level: BBa_J85800
direction: direction/forward
start: 1124
end: 1177
orientation: inline

EBFP2
type: SequenceAnnotation
persistent identity: annotation2125945
display id: annotation2125945
version: 1
top level: BBa_J85800
location: https://synbiohub.org/public/igem/BBa_J85800/annotation2125945/range2125945/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J85800/annotation2125945/range2125945/1
type: Range
persistent identity: range2125945
display id: range2125945
version: 1
top level: BBa_J85800
direction: direction/forward
start: 1178
end: 1897
orientation: inline

splice site 1
type: SequenceAnnotation
persistent identity: annotation2125946
display id: annotation2125946
version: 1
top level: BBa_J85800
location: https://synbiohub.org/public/igem/BBa_J85800/annotation2125946/range2125946/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J85800/annotation2125946/range2125946/1
type: Range
persistent identity: range2125946
display id: range2125946
version: 1
top level: BBa_J85800
direction: direction/forward
start: 1898
end: 1908
orientation: inline

miRNA FF4
type: SequenceAnnotation
persistent identity: annotation2125947
display id: annotation2125947
version: 1
top level: BBa_J85800
location: https://synbiohub.org/public/igem/BBa_J85800/annotation2125947/range2125947/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_J85800/annotation2125947/range2125947/1
type: Range
persistent identity: range2125947
display id: range2125947
version: 1
top level: BBa_J85800
direction: direction/forward
start: 1909
end: 2257
orientation: inline

splice site 2
type: SequenceAnnotation
persistent identity: annotation2125948
display id: annotation2125948
version: 1
top level: BBa_J85800
location: https://synbiohub.org/public/igem/BBa_J85800/annotation2125948/range2125948/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J85800/annotation2125948/range2125948/1
type: Range
persistent identity: range2125948
display id: range2125948
version: 1
top level: BBa_J85800
direction: direction/forward
start: 2258
end: 2278
orientation: inline

BBa_J85600
type: ComponentDefinition
persistent identity: BBa J85600
display id: BBa_J85600
version: 1
was generated by: iGEM to SBOL conversion
description: attB1 recombination site
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: lambda phage
owned by: james
owned by: myers
mutable description: attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning.
discontinued: false
dominant: false
experience: experience/None
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: attB1
sequence: https://synbiohub.org/public/igem/BBa_J85600_sequence/1

attB1
type: SequenceAnnotation
persistent identity: annotation2125966
display id: annotation2125966
version: 1
top level: BBa_J85600
location: https://synbiohub.org/public/igem/BBa_J85600/annotation2125966/range2125966/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J85600/annotation2125966/range2125966/1
type: Range
persistent identity: range2125966
display id: range2125966
version: 1
top level: BBa_J85600
direction: direction/forward
start: 2
end: 22
orientation: inline

BBa_J85701
type: ComponentDefinition
persistent identity: BBa J85701
display id: BBa_J85701
version: 1
was generated by: iGEM to SBOL conversion
description: Hef1a
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: Hef1a
owned by: james
owned by: myers
mutable description: Hef1a
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Hef1a
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: pHef1A
sequence: https://synbiohub.org/public/igem/BBa_J85701_sequence/1

pHef1A
type: SequenceAnnotation
persistent identity: annotation2125986
display id: annotation2125986
version: 1
top level: BBa_J85701
location: https://synbiohub.org/public/igem/BBa_J85701/annotation2125986/range2125986/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J85701/annotation2125986/range2125986/1
type: Range
persistent identity: range2125986
display id: range2125986
version: 1
top level: BBa_J85701
direction: direction/forward
start: 23
end: 1196
orientation: inline

BBa_J85801
type: ComponentDefinition
persistent identity: BBa J85801
display id: BBa_J85801
version: 1
was generated by: iGEM to SBOL conversion
description: rtTa3-2A-NLS-mKate2
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: various
owned by: james
owned by: myers
mutable description: rtTa3-2A-NLS-mKate2 Features: * reverse tet transactivator version 3 * 2A translational fusion tag * nuclear localization sequence fused to fluorescent protein mKate version 2
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: rtTA3
sequence annotation: 2A
sequence annotation: NLS
sequence annotation: mKate2
sequence: https://synbiohub.org/public/igem/BBa_J85801_sequence/1

rtTA3
type: SequenceAnnotation
persistent identity: annotation2125987
display id: annotation2125987
version: 1
top level: BBa_J85801
location: https://synbiohub.org/public/igem/BBa_J85801/annotation2125987/range2125987/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J85801/annotation2125987/range2125987/1
type: Range
persistent identity: range2125987
display id: range2125987
version: 1
top level: BBa_J85801
direction: direction/forward
start: 10
end: 714
orientation: inline

2A
type: SequenceAnnotation
persistent identity: annotation2125988
display id: annotation2125988
version: 1
top level: BBa_J85801
location: https://synbiohub.org/public/igem/BBa_J85801/annotation2125988/range2125988/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J85801/annotation2125988/range2125988/1
type: Range
persistent identity: range2125988
display id: range2125988
version: 1
top level: BBa_J85801
direction: direction/forward
start: 721
end: 774
orientation: inline

NLS
type: SequenceAnnotation
persistent identity: annotation2125989
display id: annotation2125989
version: 1
top level: BBa_J85801
location: https://synbiohub.org/public/igem/BBa_J85801/annotation2125989/range2125989/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J85801/annotation2125989/range2125989/1
type: Range
persistent identity: range2125989
display id: range2125989
version: 1
top level: BBa_J85801
direction: direction/forward
start: 781
end: 804
orientation: inline

mKate2
type: SequenceAnnotation
persistent identity: annotation2125990
display id: annotation2125990
version: 1
top level: BBa_J85801
location: https://synbiohub.org/public/igem/BBa_J85801/annotation2125990/range2125990/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J85801/annotation2125990/range2125990/1
type: Range
persistent identity: range2125990
display id: range2125990
version: 1
top level: BBa_J85801
direction: direction/forward
start: 805
end: 1500
orientation: inline

BBa_J85702
type: ComponentDefinition
persistent identity: BBa J85702
display id: BBa_J85702
version: 1
was generated by: iGEM to SBOL conversion
description: Hef1a-LacO1Oid
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: Sairam Subramanian
owned by: james
owned by: myers
mutable description: Hef1a-LacO1Oid Features: * Human elongation factor 1 alpha constitutive promoter * lac operator 1 * lac operator ideal
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Position of Lac operators
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Hef1a
sequence annotation: lacO1
sequence annotation: lacOid
sequence: https://synbiohub.org/public/igem/BBa_J85702_sequence/1

Hef1a
type: SequenceAnnotation
persistent identity: annotation2126000
display id: annotation2126000
version: 1
top level: BBa_J85702
location: https://synbiohub.org/public/igem/BBa_J85702/annotation2126000/range2126000/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J85702/annotation2126000/range2126000/1
type: Range
persistent identity: range2126000
display id: range2126000
version: 1
top level: BBa_J85702
direction: direction/forward
start: 16
end: 1290
orientation: inline

lacO1
type: SequenceAnnotation
persistent identity: annotation2126001
display id: annotation2126001
version: 1
top level: BBa_J85702
location: https://synbiohub.org/public/igem/BBa_J85702/annotation2126001/range2126001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J85702/annotation2126001/range2126001/1
type: Range
persistent identity: range2126001
display id: range2126001
version: 1
top level: BBa_J85702
direction: direction/forward
start: 216
end: 236
orientation: inline

lacOid
type: SequenceAnnotation
persistent identity: annotation2126002
display id: annotation2126002
version: 1
top level: BBa_J85702
location: https://synbiohub.org/public/igem/BBa_J85702/annotation2126002/range2126002/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_J85702/annotation2126002/range2126002/1
type: Range
persistent identity: range2126002
display id: range2126002
version: 1
top level: BBa_J85702
direction: direction/forward
start: 287
end: 306
orientation: inline

BBa_J85802
type: ComponentDefinition
persistent identity: BBa J85802
display id: BBa_J85802
version: 1
was generated by: iGEM to SBOL conversion
description: EYFP-FF4x4
created: 2011-09-05T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: Various
owned by: james
owned by: myers
mutable description: EYFP-FF4x4 Features: * Enhanced yellow fluorescent protein * microRNA target site FF4 (4 copies)
discontinued: false
dominant: false
group u list: _406_
m user id: 0
owner id: 106
owning group id: 406
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Number of FF4 sites
star: false
creator: Ron Weiss
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: EYFP
sequence annotation: FF4
sequence: https://synbiohub.org/public/igem/BBa_J85802_sequence/1

EYFP
type: SequenceAnnotation
persistent identity: annotation2126003
display id: annotation2126003
version: 1
top level: BBa_J85802
location: https://synbiohub.org/public/igem/BBa_J85802/annotation2126003/range2126003/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J85802/annotation2126003/range2126003/1
type: Range
persistent identity: range2126003
display id: range2126003
version: 1
top level: BBa_J85802
direction: direction/forward
start: 36
end: 755
orientation: inline

FF4
type: SequenceAnnotation
persistent identity: annotation2126004
display id: annotation2126004
version: 1
top level: BBa_J85802
location: https://synbiohub.org/public/igem/BBa_J85802/annotation2126004/range2126004/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_J85802/annotation2126004/range2126004/1
type: Range
persistent identity: range2126004
display id: range2126004
version: 1
top level: BBa_J85802
direction: direction/forward
start: 767
end: 788
orientation: inline

FF4
type: SequenceAnnotation
persistent identity: annotation2126005
display id: annotation2126005
version: 1
top level: BBa_J85802
location: https://synbiohub.org/public/igem/BBa_J85802/annotation2126005/range2126005/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_J85802/annotation2126005/range2126005/1
type: Range
persistent identity: range2126005
display id: range2126005
version: 1
top level: BBa_J85802
direction: direction/forward
start: 789
end: 810
orientation: inline

FF4
type: SequenceAnnotation
persistent identity: annotation2126006
display id: annotation2126006
version: 1
top level: BBa_J85802
location: https://synbiohub.org/public/igem/BBa_J85802/annotation2126006/range2126006/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_J85802/annotation2126006/range2126006/1
type: Range
persistent identity: range2126006
display id: range2126006
version: 1
top level: BBa_J85802
direction: direction/forward
start: 811
end: 832
orientation: inline

FF4
type: SequenceAnnotation
persistent identity: annotation2126007
display id: annotation2126007
version: 1
top level: BBa_J85802
location: https://synbiohub.org/public/igem/BBa_J85802/annotation2126007/range2126007/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_J85802/annotation2126007/range2126007/1
type: Range
persistent identity: range2126007
display id: range2126007
version: 1
top level: BBa_J85802
direction: direction/forward
start: 833
end: 854
orientation: inline

https://synbiohub.org/public/igem/BBa_J85950_sequence/1
type: Sequence
persistent identity: BBa J85950 sequence
display id: BBa_J85950_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgacccaagtttgtacaaaaaagcagtcccaccatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagagcagcctgaggcctcctaagaagaagaggaaggttgagggccgcggcagcctgctgacctgcggcgacgtggaggaaaaccccggccccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgaggggcgagggcgagggcgatgccaccaacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgagccacggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcacctacaagacccgcgccgaggtgaagttcgagggcgacaccctagtgaaccgcatcgagctgaagggcgtcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacttcaacagccacaacatctatatcatggccgtcaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85900_sequence/1
type: Sequence
persistent identity: BBa J85900 sequence
display id: BBa_J85900_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgacccaagtttgtacaaaaaagcagtcccaccatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagagcagcctgaggcctcctaagaagaagaggaaggttgagggccgcggcagcctgctgacctgcggcgacgtggaggaaaaccccggccccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgaggggcgagggcgagggcgatgccaccaacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgagccacggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcacctacaagacccgcgccgaggtgaagttcgagggcgacaccctagtgaaccgcatcgagctgaagggcgtcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacttcaacagccacaacatctatatcatggccgtcaag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85901_sequence/1
type: Sequence
persistent identity: BBa J85901 sequence
display id: BBa_J85901_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctccgaattcgcccttattaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttgtggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtggatcccaagggcgaattcgacccaagtttgtacaaaaaagcaggacgccaccatgtctaggctggacaagagcaaagtcataaacggagctctggaattactcaatggtgtcggtatcgaaggcctgacgacaaggaaactcgctcaaaagctgggagttgagcagcctaccctgtactggcacgtgaagaacaagcgggccctgctcgatgccctgccaatcgagatgctggacaggcatcatacccacttctgccccctggaaggcgagtcatggcaagactttctgcggaacaacgccaagtcataccgctgtgctctcctctcacatcgcgacggggctaaagtgcatctcggcacccgcccaacagagaaacagtacgaaaccctggaaaatcagctcgcgttcctgtgtcagcaaggcttctccctggagaacgcactgtacgctctgtccgccgtgggccactttacactgggctgcgtattggaggaacaggagcatcaagtagcaaaagaggaaagagagacacctaccaccgattctatgcccccacttctgagacaagcaattgagctgttcgaccggcagggagccgaacctgccttccttttcggcctggaactaatcatatgtggcctggagaaacagctaaagtgcgaaagcggcgggccgaccgacgcccttgacgattttgacttagacatgctcccagccgatgcccttgacgattttgaccttgacatgctccccggggaattggagggccgcggcagcctgctgacctgcggcgacgtggagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85902_sequence/1
type: Sequence
persistent identity: BBa J85902 sequence
display id: BBa_J85902_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctccgaattctaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaagctagaattgtgagcggataacaattccacagtcgaccctaggttgtgtcgcgagtgttggatcccagctgacaccaattgtgagcgctcacaattgctagccgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgaagacttgatccccggaattcgacccaagtttgtacaaaaaagcagttcttccatttcaggtggatccaccggtcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccaagctgagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85602_sequence/1
type: Sequence
persistent identity: BBa J85602 sequence
display id: BBa_J85602_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttaccgcatctagacctaactgagatactgccatagacgactac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85603_sequence/1
type: Sequence
persistent identity: BBa J85603 sequence
display id: BBa_J85603_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccatccctctgggccttagatagccggatacagtgactttgaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85700_sequence/1
type: Sequence
persistent identity: BBa J85700 sequence
display id: BBa_J85700_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85800_sequence/1
type: Sequence
persistent identity: BBa J85800 sequence
display id: BBa_J85800_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccaccatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagagcagcctgaggcctcctaagaagaagaggaaggttgagggccgcggcagcctgctgacctgcggcgacgtggaggaaaaccccggccccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgaggggcgagggcgagggcgatgccaccaacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgagccacggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcacctacaagacccgcgccgaggtgaagttcgagggcgacaccctagtgaaccgcatcgagctgaagggcgtcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaacttcaacagccacaacatctatatcatggccgtcaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacgtggaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacagccactacctgagcacccagtccgtgctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttccgcaccgccgccgggatcactctcggcatggacgagctgtacaagtaagaggtgagtatgtgctcgcttcggcagcacatatactatgttgaatgaggcttcagtactttacagaatcgttgcctgcacatcttggaaacacttgctggga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85600_sequence/1
type: Sequence
persistent identity: BBa J85600 sequence
display id: BBa_J85600_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaagtttgtacaaaaaagcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85701_sequence/1
type: Sequence
persistent identity: BBa J85701 sequence
display id: BBa_J85701_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctccgaattcgcccttattaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttgtggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtggatcccaagggcgaattcgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85801_sequence/1
type: Sequence
persistent identity: BBa J85801 sequence
display id: BBa_J85801_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgccaccatgtctaggctggacaagagcaaagtcataaacggagctctggaattactcaatggtgtcggtatcgaaggcctgacgacaaggaaactcgctcaaaagctgggagttgagcagcctaccctgtactggcacgtgaagaacaagcgggccctgctcgatgccctgccaatcgagatgctggacaggcatcatacccacttctgccccctggaaggcgagtcatggcaagactttctgcggaacaacgccaagtcataccgctgtgctctcctctcacatcgcgacggggctaaagtgcatctcggcacccgcccaacagagaaacagtacgaaaccctggaaaatcagctcgcgttcctgtgtcagcaaggcttctccctggagaacgcactgtacgctctgtccgccgtgggccactttacactgggctgcgtattggaggaacaggagcatcaagtagcaaaagaggaaagagagacacctaccaccgattctatgcccccacttctgagacaagcaattgagctgttcgaccggcagggagccgaacctgccttccttttcggcctggaactaatcatatgtggcctggagaaacagctaaagtgcgaaagcggcgggccgaccgacgcccttgacgattttgacttagacatgctcccagccgatgcccttgacgattttgaccttgacatgctccccggggaattggagggccgcggcagcctgctgacctgcggcgacgtggaggaaaaccccggccccatggtgccaccaaaaaaaaaacgcaaagttgtgagcgagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggcggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccccgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagaccctgtaccccgctgacggcggcctggaaggcagagccgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacagatgatacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85702_sequence/1
type: Sequence
persistent identity: BBa J85702 sequence
display id: BBa_J85702_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctccgaattctaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaagctagaattgtgagcggataacaattccacagtcgaccctaggttgtgtcgcgagtgttggatcccagctgacaccaattgtgagcgctcacaattgctagccgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgaagacttgatccccggaattcgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J85802_sequence/1
type: Sequence
persistent identity: BBa J85802 sequence
display id: BBa_J85802_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcttccatttcaggtggatccaccggtcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaagcggcgcgccccgcttgaagtctttaattaaaccgcttgaagtctttaattaaaccgcttgaagtctttaattaaaccgcttgaagtctttaattaaagctagt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z