TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4-scar2-rtTa3-2A-NLS-mKate2-scar3-EYFP-FF4x4 Composite part that forms an mammalian LacI inverter regulated by TRE
Hef1a-B1-rtTA3-2A-NLS-mKate2 Constitutive promoter Hef1a regulates production of rtTA3 transcriptional activator and nuclear-localized FP mKate
Hef1a-LacO1Oid-B1-EYFP-FF4x4 Features: * Human elongation factor 1 alpha constitutive promoter * Lac operator 1 * Lac operator ideal * (attB1 scar from recombination assembly) * Enhanced yellow FP * microRNA target site FF4 (four copies)
Tet response element; tightly regulated.
LacI-T2A-EBFP2-FF5-miRFF4 Features: * transcriptional repressor LacI * 2A translational fusion tag * enhanced blue fluorescent protein EBFP2 * microRNA target site FF5 * microRNA FF4
attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning.
rtTa3-2A-NLS-mKate2 Features: * reverse tet transactivator version 3 * 2A translational fusion tag * nuclear localization sequence fused to fluorescent protein mKate version 2
Hef1a-LacO1Oid Features: * Human elongation factor 1 alpha constitutive promoter * lac operator 1 * lac operator ideal
EYFP-FF4x4 Features: * Enhanced yellow fluorescent protein * microRNA target site FF4 (4 copies)
Conversion of the iGEM parts registry to SBOL2.1